Metabolic flux analysis of the halophilic archaeon Haladaptatus paucihalophilus
文献类型：期刊论文
| 作者 | Liu, Guangxiu1,2; Zhang, Manxiao1,2; Mo, Tianlu3; He, Lian4; Zhang, Wei1,2; Yu, Yi4; Zhang, Qi3; Ding, Wei1,2,3 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2015-11-27 |
| 卷号 | 467期号:4页码:1058-1062 |
| ISSN号 | 0006-291X |
| 关键词 | C-13-assisted metabolism analysis Glycine biosynthesis Halophilic archaea Isoleucine biosynthesis Lysine biosynthesis |
| DOI | 10.1016/j.bbrc.2015.09.174 |
| 通讯作者 | Zhang, Qi(qizhang@sioc.ac.cn) |
| 英文摘要 | This work reports the C-13-assisted metabolic flux analysis of Haladaptatus paucihalophilus, a halophilic archaeon possessing an intriguing osmoadaption mechanism. We showed that the carbon flow is through the oxidative tricarboxylic acid (TCA) cycle whereas the reductive TCA cycle is not operative in H. paucihalophilus. In addition, both threonine and the citramalate pathways contribute to isoleucine biosynthesis, whereas lysine is synthesized through the diaminopimelate pathway and not through the alpha-aminoadipate pathway. Unexpected, the labeling patterns of glycine from the cells grown on [1-C-13] pyruvate and [2-C-13]pyruvate suggest that, unlike all the organisms investigated so far, in which glycine is produced exclusively from the serine hydroxymethyltransferase (SHMT) pathway, glycine biosynthesis in H. paucihalophilus involves different pathways including SHMT, threonine aldolase (TA) and the reverse reaction of glycine cleavage system (GCS), demonstrating for the first time that other pathways instead of SHMT can also make a significant contribution to the cellular glycine pool. Transcriptional analysis confirmed that both TA and GCS genes were transcribed in H. paucihalophilus, and the transcriptional level is independent of salt concentrations in the culture media. This study expands our understanding of amino acid biosynthesis and provides valuable insights into the metabolism of halophilic archaea. (C) 2015 Elsevier Inc. All rights reserved. |
| 收录类别 | SCI |
| WOS关键词 | CHROMATOGRAPHY-MASS SPECTROMETRY ; GC-MS ; BIOSYNTHESIS ; GLYCINE ; BETAINE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| WOS记录号 | WOS:000365145300070 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2557198 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Zhang, Qi |
| 作者单位 | 1.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Key Lab Desert & Desertificat, Lanzhou 730000, Peoples R China 2.Key Lab Extreme Environm Microbial Resources & En, Lanzhou 730000, Gansu, Peoples R China 3.Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China 4.Wuhan Univ, Key Lab Combinatory Biosynth & Drug Discovery, Sch Pharmaceut Sci, Minist Educ, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Guangxiu,Zhang, Manxiao,Mo, Tianlu,et al. Metabolic flux analysis of the halophilic archaeon Haladaptatus paucihalophilus[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,467(4):1058-1062. |
| APA | Liu, Guangxiu.,Zhang, Manxiao.,Mo, Tianlu.,He, Lian.,Zhang, Wei.,...&Ding, Wei.(2015).Metabolic flux analysis of the halophilic archaeon Haladaptatus paucihalophilus.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,467(4),1058-1062. |
| MLA | Liu, Guangxiu,et al."Metabolic flux analysis of the halophilic archaeon Haladaptatus paucihalophilus".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 467.4(2015):1058-1062. |
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来源：寒区旱区环境与工程研究所
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