Development of a luciferase-based reporter of transcriptional gene silencing that enables bidirectional mutant screening in Arabidopsis thaliana
文献类型：期刊论文
| 作者 | Won,So Youn1; Li,Shengben1; Zheng,Binglian1,7; Zhao,Yuanyuan1; Li,Dongming1,2; Zhao,Xin1,3; Yi,Huilan1,4; Gao,Lei1; Dinh,Thanh Theresa1,5; Chen,Xuemei1,6 |
| 刊名 | Silence
 |
| 出版日期 | 2012-06-07 |
| 卷号 | 3期号:1 |
| 关键词 | Cytosine methylation Demethylation RdDM MOM1 Transcriptional gene silencing siRNA |
| ISSN号 | 1758-907X |
| DOI | 10.1186/1758-907X-3-6 |
| 通讯作者 | Chen,Xuemei(xuemei.chen@ucr.edu) |
| 英文摘要 | AbstractBackgroundCytosine methylation is an important chromatin modification that maintains genome integrity and regulates gene expression through transcriptional gene silencing. Major players in de novo methylation guided by siRNAs (known as RNA-directed DNA methylation, or RdDM), maintenance methylation, and active demethylation have been identified in Arabidopsis. However, active demethylation only occurs at a subset of RdDM loci, raising the question of how the homeostasis of DNA methylation is achieved at most RdDM loci. To identify factors that regulate the levels of cytosine methylation, we aimed to establish a transgenic reporter system that allows for forward genetic screens in Arabidopsis.ResultsWe introduced a dual 35 S promoter (d35S) driven luciferase reporter, LUCH, into Arabidopsis and isolated a line with a moderate level of luciferase activity. LUCH produced transgene-specific 24 nucleotide siRNAs and its d35S contained methylated cytosine in CG, CHG and CHH contexts. Treatment of the transgenic line with an inhibitor of cytosine methylation de-repressed luciferase activity. Mutations in several components of the RdDM pathway but not the maintenance methylation genes resulted in reduced d35S methylation, especially CHH methylation, and de-repression of luciferase activity. A mutation in MOM1, which is known to cooperate with RdDM to silence transposons, reduced d35S DNA methylation and de-repressed LUCH expression. A mutation in ROS1, a cytosine demethylation enzyme, increased d35S methylation and reduced LUCH expression.ConclusionWe developed a luciferase-based reporter, LUCH, which reports both DNA methylation directed by small RNAs and active demethylation by ROS1 in Arabidopsis. The moderate basal level of LUCH expression allows for bi-directional genetic screens that dissect the mechanisms of DNA methylation as well as demethylation. |
| 收录类别 | BMC |
| 语种 | 英语 |
| WOS记录号 | BMC:10.1186/1758-907X-3-6 |
| 出版者 | BioMed Central |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2557246 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Chen,Xuemei |
| 作者单位 | 1.University of California; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology 2.Lanzhou University; School of Life Sciences 3.Chinese Academy of Sciences; Laboratory of Plant Stress Ecophysiology and Biotechnology, Cold and Arid Regions Environmental and Engineering Research Institute 4.Shanxi University; School of Life Science 5.University of California; NSF ChemGen IGERT program 6.University of California; Howard Hughes Medical Institute 7.Fudan University; State Key Laboratory of Genetic Engineering and Institute of Plant Biology, School of Life Sciences |
| 推荐引用方式 GB/T 7714 | Won,So Youn,Li,Shengben,Zheng,Binglian,et al. Development of a luciferase-based reporter of transcriptional gene silencing that enables bidirectional mutant screening in Arabidopsis thaliana[J]. Silence,2012,3(1). |
| APA | Won,So Youn.,Li,Shengben.,Zheng,Binglian.,Zhao,Yuanyuan.,Li,Dongming.,...&Chen,Xuemei.(2012).Development of a luciferase-based reporter of transcriptional gene silencing that enables bidirectional mutant screening in Arabidopsis thaliana.Silence,3(1). |
| MLA | Won,So Youn,et al."Development of a luciferase-based reporter of transcriptional gene silencing that enables bidirectional mutant screening in Arabidopsis thaliana".Silence 3.1(2012). |
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来源：寒区旱区环境与工程研究所
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