Purification and immuno-gold labeling of lily mottle virus from lily leaves
文献类型：期刊论文
| 作者 | Zhang, Yubao1; Wang, Yajun1; Xie, Zhongkui1; Wang, Ruoyu1; Guo, Zhihong1; van der Werf, Wopke2; Wang, Le1 |
| 刊名 | JOURNAL OF VIROLOGICAL METHODS
 |
| 出版日期 | 2016-06-01 |
| 卷号 | 232页码:33-38 |
| 关键词 | Lily mottle virus Purification Transmission electron microscope Immuno-gold labeling assay |
| ISSN号 | 0166-0934 |
| DOI | 10.1016/j.jviromet.2016.02.001 |
| 通讯作者 | Xie, Zhongkui(wxhcas@lzb.ac.cn) |
| 英文摘要 | Lily mottle virus (LMoV) is prevalent in Lilium species worldwide causing dwarfing, flower breaking, and reduced bulb yield. In this paper, an easy to use and efficient procedure is described for purification of LMoV from lily leaves. The resulting sample is characterized by a 260/280 nm absorbance ratio of 1.20 at a concentration of 1.27 mg/ml. The procedure results in high protein purity and particle integrity as shown by UV-spectrophotometry, polyacrylamide gel electrophoresis (PAGE), Western blotting, reverse transcriptase (RT)-PCR and transmission electron microscopy (TEM) in combination with immuno-gold labeling. This is the first time that an immuno-gold labeling (IGL) assay was performed to identify a virus of lily. Purified products can be used as a source of antigen in the preparation of antibodies against LMoV and may assist in the development of a diagnostic test for LMoV and in epidemiological surveys. (C) 2016 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| WOS关键词 | DAVIDII VAR. UNICOLOR ; SYMPTOMLESS VIRUS ; PLANT-VIRUSES ; LILIUM ; IDENTIFICATION ; ANTIBODY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000374365000007 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2557300 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Xie, Zhongkui |
| 作者单位 | 1.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, 320 Dong Gang West Rd, Lanzhou 730000, Peoples R China 2.Wageningen Univ, Dept Plant Sci, Ctr Crop Syst Anal, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands |
| 推荐引用方式 GB/T 7714 | Zhang, Yubao,Wang, Yajun,Xie, Zhongkui,et al. Purification and immuno-gold labeling of lily mottle virus from lily leaves[J]. JOURNAL OF VIROLOGICAL METHODS,2016,232:33-38. |
| APA | Zhang, Yubao.,Wang, Yajun.,Xie, Zhongkui.,Wang, Ruoyu.,Guo, Zhihong.,...&Wang, Le.(2016).Purification and immuno-gold labeling of lily mottle virus from lily leaves.JOURNAL OF VIROLOGICAL METHODS,232,33-38. |
| MLA | Zhang, Yubao,et al."Purification and immuno-gold labeling of lily mottle virus from lily leaves".JOURNAL OF VIROLOGICAL METHODS 232(2016):33-38. |
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来源：寒区旱区环境与工程研究所
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