Nucleoside-linked shunt products in the reaction catalyzed by the class C radical S-adenosylmethionine methyltransferase NosN
文献类型：期刊论文
| 作者 | Ding, Wei1,2,3; Wu, Yujie1,2; Ji, Xinjian2; Qianzhu, Haocheng2; Chen, Fener2; Deng, Zixin4; Yu, Yi4; Zhang, Qi2 |
| 刊名 | CHEMICAL COMMUNICATIONS
 |
| 出版日期 | 2017-05-07 |
| 卷号 | 53期号:37页码:5235-5238 |
| ISSN号 | 1359-7345 |
| DOI | 10.1039/c7cc02162c |
| 通讯作者 | Zhang, Qi(qizhang@sioc.ac.cn) |
| 英文摘要 | NosN is a class C radical S-adenosylmethionine (SAM) methyltransferase (RSMT) involved in the biosynthesis of nosiheptide, a clinically interesting thiopeptide antibiotic produced by Streptomyces actuosus. NosN employs an unprecedented catalytic mechanism, in which SAM is converted to 5'-methylthioadenosine (MTA) as a direct methyl donor. In this study, we report identification of several nucleosidelinked shunt products in the NosN-catalyzed reaction. Comparative analysis of NosN and the class A RSMT RlmN and further density functional theory (DFT) calculations reveal important mechanistic insights into the catalyses of the two types of enzymes, showing that the radical intermediates generated by similar pathways can have very diverse reactivities. This investigation provides strong evidence supporting the previous mechanistic proposal of NosN catalysis, validating the presence of a key radical adduct that results from the addition of an MTA-derived methylene radical onto the C4 of the indolyl substrate. |
| 收录类别 | SCI |
| WOS关键词 | TRYPTOPHAN LYASE NOSL ; L-METHIONINE ENZYME ; DEPENDENT METHYLTRANSFERASES ; SAM ENZYME ; MECHANISTIC INSIGHTS ; METHYL TRANSFER ; BIOSYNTHESIS ; RLMN ; MUTAGENESIS ; ANALOGS |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000400858400040 |
| 出版者 | ROYAL SOC CHEMISTRY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2557553 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Zhang, Qi |
| 作者单位 | 1.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China 2.Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China 3.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Key Lab Extreme Environm Microbial Resources & En, Lanzhou 730000, Peoples R China 4.Wuhan Univ, Sch Pharmaceut Sci, Key Lab Combinatory Biosynth & Drug Discovery, Minist Educ, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Wei,Wu, Yujie,Ji, Xinjian,et al. Nucleoside-linked shunt products in the reaction catalyzed by the class C radical S-adenosylmethionine methyltransferase NosN[J]. CHEMICAL COMMUNICATIONS,2017,53(37):5235-5238. |
| APA | Ding, Wei.,Wu, Yujie.,Ji, Xinjian.,Qianzhu, Haocheng.,Chen, Fener.,...&Zhang, Qi.(2017).Nucleoside-linked shunt products in the reaction catalyzed by the class C radical S-adenosylmethionine methyltransferase NosN.CHEMICAL COMMUNICATIONS,53(37),5235-5238. |
| MLA | Ding, Wei,et al."Nucleoside-linked shunt products in the reaction catalyzed by the class C radical S-adenosylmethionine methyltransferase NosN".CHEMICAL COMMUNICATIONS 53.37(2017):5235-5238. |
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来源：寒区旱区环境与工程研究所
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