Diversity of crude oil-degrading bacteria and alkane hydroxylase (alkB) genes from the Qinghai-Tibet Plateau
文献类型：期刊论文
| 作者 | Long, Haozhi1,2,3; Wang, Yilin1; Chang, Sijing3,4; Liu, Guangxiu2,3; Chen, Tuo3,4; Huo, Guanghua1; Zhang, Wei2,3; Wu, Xiukun2,3; Tai, Xisheng2,3; Sun, Likun2,3 |
| 刊名 | ENVIRONMENTAL MONITORING AND ASSESSMENT
 |
| 出版日期 | 2017-03-01 |
| 卷号 | 189期号:3页码:14 |
| 关键词 | Qinghai-Tibet Plateau Crude oil enrichment Alkane hydrolase gene Diversity |
| ISSN号 | 0167-6369 |
| DOI | 10.1007/s10661-017-5798-5 |
| 通讯作者 | Liu, Guangxiu(liugx@lzb.ac.cn) ; Chen, Tuo(chentuo@lzb.ac.cn) |
| 英文摘要 | The aim of this study was to survey the response of the microbial community to crude oil and the diversity of alkane hydroxylase (alkB) genes in soil samples from the Qinghai-Tibet Plateau (QTP). The enrichment cultures and clone libraries were used. Finally, 53 isolates and 94 alkB sequences were obtained from 10 pristine soil samples after enrichment at 10 degrees C with crude oil as sole carbon source. The isolates fell into the phyla Proteobacteria, Actinobacteria, and Bacteroidetes, with the dominance of Pseudomonas and Acinetobacter. The composition of degraders was different from polar habitats where Acinetobacter sp. is not a predominant responder of alkane degradative microbial communities. Phylogenetic analysis showed that the alkB genes from isolates and enrichment communities formed eight clusters and mainly related with alkB genes of Pseudomonas, Rhodococcus, and Acinetobacter. The alkB gene diversity in the QTP was lower than marine environments and polar soil samples. In particular, a total of 10 isolates exhibiting vigorous growth with crude oil could detect no crude oil degradation-related gene sequences, such as alkB, P450, almA, ndoB, and xylE genes. The Shannon-Wiener index of the alkB clone libraries from the QTP ranged from 1.00 to 2.24 which is similar with polar pristine soil samples but lower than that of contaminated soils. These results indicated that the Pseudomonas, Acinetobacter, and Rhodococcus genera are the candidate for in situ bioremediation, and the environment of QTP may be still relatively uncontaminated by crude oil. |
| 收录类别 | SCI |
| WOS关键词 | MICROBIAL COMMUNITIES ; MONOOXYGENASE GENES ; DEGRADATION ; CONSORTIUM ; SOILS ; ENVIRONMENTS ; MICROORGANISMS ; GRADIENT ; REGION ; ENZYME |
| WOS研究方向 | Environmental Sciences & Ecology |
| WOS类目 | Environmental Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000395000800023 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2557586 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Liu, Guangxiu; Chen, Tuo |
| 作者单位 | 1.Jiangxi Agr Univ, Coll Biosci & Bioengn, Nanchang 330045, Jiangxi, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Key Lab Desert & Desertificat, Lanzhou 730000, Peoples R China 3.Key Lab Extreme Environm Microbial Resources & En, Lanzhou 730000, Gansu, Peoples R China 4.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, State Key Lab Cryospher Sci, Lanzhou, Gansu 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Long, Haozhi,Wang, Yilin,Chang, Sijing,et al. Diversity of crude oil-degrading bacteria and alkane hydroxylase (alkB) genes from the Qinghai-Tibet Plateau[J]. ENVIRONMENTAL MONITORING AND ASSESSMENT,2017,189(3):14. |
| APA | Long, Haozhi.,Wang, Yilin.,Chang, Sijing.,Liu, Guangxiu.,Chen, Tuo.,...&Zhang, Baogui.(2017).Diversity of crude oil-degrading bacteria and alkane hydroxylase (alkB) genes from the Qinghai-Tibet Plateau.ENVIRONMENTAL MONITORING AND ASSESSMENT,189(3),14. |
| MLA | Long, Haozhi,et al."Diversity of crude oil-degrading bacteria and alkane hydroxylase (alkB) genes from the Qinghai-Tibet Plateau".ENVIRONMENTAL MONITORING AND ASSESSMENT 189.3(2017):14. |
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来源：寒区旱区环境与工程研究所
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