A trehalose biosynthetic enzyme doubles as an osmotic stress sensor to regulate bacterial morphogenesis
文献类型：期刊论文
| 作者 | Chen, Ximing1,2,3; An, Lizhe1; Fan, Xiaochuan1; Ju, Furong1; Zhang, Binglin2,3; Sun, Haili4; Xiao, Jianxi5; Hu, Wei1; Qu, Tao1; Guan, Liping1 |
| 刊名 | PLOS GENETICS
 |
| 出版日期 | 2017-10-01 |
| 卷号 | 13期号:10页码:19 |
| ISSN号 | 1553-7404 |
| DOI | 10.1371/journal.pgen.1007062 |
| 通讯作者 | Dyson, Paul(p.j.dyson@swansea.ac.uk) |
| 英文摘要 | The dissacharide trehalose is an important intracellular osmoprotectant and the OtsA/B pathway is the principal pathway for trehalose biosynthesis in a wide range of bacterial species. Scaffolding proteins and other cytoskeletal elements play an essential role in morphogenetic processes in bacteria. Here we describe how OtsA, in addition to its role in trehalose biosynthesis, functions as an osmotic stress sensor to regulate cell morphology in Arthrobacter strain A3. In response to osmotic stress, this and other Arthrobacter species undergo a transition from bacillary to myceloid growth. An otsA null mutant exhibits constitutive myceloid growth. Osmotic stress leads to a depletion of trehalose-6-phosphate, the product of the OtsA enzyme, and experimental depletion of this metabolite also leads to constitutive myceloid growth independent of OtsA function. In vitro analyses indicate that OtsA can self-assemble into protein networks, promoted by trehalose-6-phosphate, a property that is not shared by the equivalent enzyme from E. coli, despite the latter's enzymatic activity when expressed in Arthrobacter. This, and the localization of the protein in non-stressed cells at the mid-cell and poles, indicates that OtsA from Arthrobacter likely functions as a cytoskeletal element regulating cell morphology. Recruiting a biosynthetic enzyme for this morphogenetic function represents an intriguing adaptation in bacteria that can survive in extreme environments. |
| 收录类别 | SCI |
| WOS关键词 | CELL-WALL SYNTHESIS ; ESCHERICHIA-COLI ; TREHALOSE-6-PHOSPHATE SYNTHASE ; ARTHROBACTER-GLOBIFORMIS ; STREPTOMYCES-COELICOLOR ; POLAR GROWTH ; CTP SYNTHASE ; DIVISION ; MREB ; FILAMENTS |
| WOS研究方向 | Genetics & Heredity |
| WOS类目 | Genetics & Heredity |
| 语种 | 英语 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| WOS记录号 | WOS:000414161300028 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2557644 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Dyson, Paul |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Desert & Desertificat, Northwest Inst Ecoenvironm & Resources, Lanzhou, Gansu, Peoples R China 2.Lanzhou Univ, State Key Lab Arid & Grassland Agroecol, Minist Educ, Lanzhou, Gansu, Peoples R China 3.Key Lab Extreme Environm Microbial Resources & En, Lanzhou, Gansu, Peoples R China 4.Lanzhou City Univ, Sch Chem & Environm Sci, Lanzhou, Gansu, Peoples R China 5.Lanzhou Univ, State Key Lab Appl Organ Chem, Coll Chem & Chem Engn, Lanzhou, Gansu, Peoples R China 6.Yunnan Univ, Key Lab Microbial Divers Southwest China, Minist Educ, Kunming, Yunnan, Peoples R China 7.Yunnan Univ, Lab Conservat & Utilizat Bioresources, Yunnan Inst Microbiol, Kunming, Yunnan, Peoples R China 8.Swansea Univ, Med Sch, Inst Life Sci, Swansea, W Glam, Wales |
| 推荐引用方式 GB/T 7714 | Chen, Ximing,An, Lizhe,Fan, Xiaochuan,et al. A trehalose biosynthetic enzyme doubles as an osmotic stress sensor to regulate bacterial morphogenesis[J]. PLOS GENETICS,2017,13(10):19. |
| APA | Chen, Ximing.,An, Lizhe.,Fan, Xiaochuan.,Ju, Furong.,Zhang, Binglin.,...&Dyson, Paul.(2017).A trehalose biosynthetic enzyme doubles as an osmotic stress sensor to regulate bacterial morphogenesis.PLOS GENETICS,13(10),19. |
| MLA | Chen, Ximing,et al."A trehalose biosynthetic enzyme doubles as an osmotic stress sensor to regulate bacterial morphogenesis".PLOS GENETICS 13.10(2017):19. |
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来源：寒区旱区环境与工程研究所
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