A Comet Assay for DNA Damage and Repair After Exposure to Carbon-Ion Beams or X-rays in Saccharomyces Cerevisiae
文献类型：期刊论文
| 作者 | Zhang, Miaomiao1,2,3; Cao, Guozhen4; Guo, Xiaopeng1,2; Gao, Yue1,2; Li, Wenjian1,3; Lu, Dong1,3 |
| 刊名 | DOSE-RESPONSE
 |
| 出版日期 | 2018-08-09 |
| 卷号 | 16期号:3页码:9 |
| ISSN号 | 1559-3258 |
| 关键词 | DNA damage comet assay DNA repair model fitting Saccharomyces cerevisiae |
| DOI | 10.1177/1559325818792467 |
| 通讯作者 | Lu, Dong(ld@impcas.ac.cn) |
| 英文摘要 | Ionizing radiation (IR) can result in serious genomic instability and genotoxicity by causing DNA damage. Carbon ion (CI) beams and X-rays are typical IRs and possess high-linear energy transfer (LET) and low-LET, respectively. In this article, a comet assay that was optimized by decreasing the electrophoresis time (8 minutes) and voltage (0.5 V/cm) was performed to elucidate and quantify the DNA damage induced by CI or X-rays radiation. Two quantitative methods for the comet assay, namely, comet score and olive tail moment, were compared, and the appropriate means and parameter values were selected for the present assay. The dose-effect relationship for CI or X-rays radiation and the DNA repair process were studied in yeast cells. These results showed that the quadratic function fitted the dose-effect relationship after CI or X-rays exposure, and the trend for the models fitted the dose-effect curves for various repair times was precisely described by the cubic function. A kinetics model was also creatively used to describe the process of DNA repair, and equations were calculated within repairable ranges that could be used to roughly evaluate the process and time necessary for DNA repair. |
| 收录类别 | SCI |
| WOS关键词 | DOUBLE-STRAND BREAKS ; HIGH-LET IRRADIATION ; YEAST-CELLS ; GEL-ELECTROPHORESIS ; OXIDATIVE DAMAGE ; RADIATION ; GAMMA ; GENOTOXICITY ; ORGANS ; WATER |
| WOS研究方向 | Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging ; Toxicology |
| WOS类目 | Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging ; Toxicology |
| 语种 | 英语 |
| 出版者 | SAGE PUBLICATIONS INC |
| WOS记录号 | WOS:000441279800001 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2557783 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Lu, Dong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 3.Key Lab Microbial Resources Exploitat & Applicat, Lanzhou, Gansu, Peoples R China 4.Xinjiang Med Univ, Dept Pharmacol, Sch Preclin Med, Urumqi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Miaomiao,Cao, Guozhen,Guo, Xiaopeng,et al. A Comet Assay for DNA Damage and Repair After Exposure to Carbon-Ion Beams or X-rays in Saccharomyces Cerevisiae[J]. DOSE-RESPONSE,2018,16(3):9. |
| APA | Zhang, Miaomiao,Cao, Guozhen,Guo, Xiaopeng,Gao, Yue,Li, Wenjian,&Lu, Dong.(2018).A Comet Assay for DNA Damage and Repair After Exposure to Carbon-Ion Beams or X-rays in Saccharomyces Cerevisiae.DOSE-RESPONSE,16(3),9. |
| MLA | Zhang, Miaomiao,et al."A Comet Assay for DNA Damage and Repair After Exposure to Carbon-Ion Beams or X-rays in Saccharomyces Cerevisiae".DOSE-RESPONSE 16.3(2018):9. |
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来源：寒区旱区环境与工程研究所
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