Methylation of promoter of RBL1 enhances the radioresistance of three dimensional cultured carcinoma cells
文献类型：期刊论文
| 作者 | Pan, Dong1,2,3; Chen, Yaxiong1,2; Du, Yarong1,2; Ren, Zhenxin1,2; Li, Xiaoman1,2,3; Hu, Burong1,2,4,5 |
| 刊名 | ONCOTARGET
 |
| 出版日期 | 2017 |
| 卷号 | 8期号:3页码:4422-4435 |
| 关键词 | three dimensional cultured cells radioresistance methylation promoter RBL1 |
| ISSN号 | 1949-2553 |
| DOI | 10.18632/oncotarget.12647 |
| 通讯作者 | Hu, Burong(hubr@impcas.ac.cn) |
| 英文摘要 | Three dimensional (3D) culture in vitro is a new cell culture model that more closely mimics the physiology features of the in vivo environment and is being used widely in the field of medical and biological research. It has been demonstrated that cancer cells cultured in 3D matrices are more radioresistant compared with cells in monolayer (2D). However, the mechanisms causing this difference remain largely unclear. Here we found that the cell cycle distribution and expression of cell cycle regulation genes in 3D A549 cells are different from the 2D. The higher levels of the promotor methylation of cell cycle regulation genes such as RBL1 were observed in 3D A549 cells compared with cells in 2D. The treatments of irradiation or 5-Aza-CdR activated the demethylation of RBL1 promotor and resulted in the increased expression of RBL1 only in 3D A549 cells. Inhibition of RBL1 enhanced the radioresistance and decreased the G2/M phase arrest induced by irradiation in 2D A549 and MCF7 cells. Overexpression of RBL1 sensitized 3D cultured A549 and MCF7 cells to irradiation. Taken together, to our knowledge, it is the first time to revealthat the low expression of RBL1 due to itself promotor methylation in 3D cells enhances the radioresistance. Our finding sheds a new light on understanding the features of the 3D cultured cell model and its application in basic research into cancer radiotherapy and medcine development. |
| 收录类别 | SCI |
| WOS关键词 | MAMMARY EPITHELIAL-CELLS ; DNA METHYLATION ; GENE-EXPRESSION ; EXTRACELLULAR-MATRIX ; RADIATION SENSITIVITY ; TISSUE ARCHITECTURE ; CANCER STATISTICS ; BREAST-CANCER ; P107 PROTEIN ; HYPERMETHYLATION |
| WOS研究方向 | Oncology ; Cell Biology |
| WOS类目 | Oncology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000393228400056 |
| 出版者 | IMPACT JOURNALS LLC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2558000 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Hu, Burong |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Key Lab Space Radiobiol Gansu Prov, Lanzhou, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Soochow Univ, Collaborat Innovat Ctr Radiat Med Jiangsu Higher, Suzhou, Jiangsu, Peoples R China 5.Soochow Univ, Sch Radiol & Interdisciplinary Sci RAD X, Suzhou, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pan, Dong,Chen, Yaxiong,Du, Yarong,et al. Methylation of promoter of RBL1 enhances the radioresistance of three dimensional cultured carcinoma cells[J]. ONCOTARGET,2017,8(3):4422-4435. |
| APA | Pan, Dong,Chen, Yaxiong,Du, Yarong,Ren, Zhenxin,Li, Xiaoman,&Hu, Burong.(2017).Methylation of promoter of RBL1 enhances the radioresistance of three dimensional cultured carcinoma cells.ONCOTARGET,8(3),4422-4435. |
| MLA | Pan, Dong,et al."Methylation of promoter of RBL1 enhances the radioresistance of three dimensional cultured carcinoma cells".ONCOTARGET 8.3(2017):4422-4435. |
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来源：寒区旱区环境与工程研究所
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