Heavy-ion mutagenesis significantly enhances enduracidin production by Streptomyces fungicidicus
文献类型：期刊论文
| 作者 | Liu, Lu1,2; Hu, Wei1; Li, Wen-jian1; Wang, Shu-yang1; Lu, Dong1; Tian, Xue-jiao1,2; Mao, Yan-qin1,2; Liu, Jing1; Chen, Ji-hong1 |
| 刊名 | ENGINEERING IN LIFE SCIENCES
 |
| 出版日期 | 2019-02-01 |
| 卷号 | 19期号:2页码:112-120 |
| 关键词 | fermentation industry heavy ion mutagenesis mutation tool screening process Streptomyces fungicidicus |
| ISSN号 | 1618-0240 |
| DOI | 10.1002/elsc.201800109 |
| 通讯作者 | Hu, Wei(hudongli007@impcas.ac.cn) ; Chen, Ji-hong(chjh@impcas.ac.cn) |
| 英文摘要 | To improve fermentative production of enduracidin, heavy-ion beams generated by the Heavy Ion Research Facility in Lanzhou (HIRFL), China, were employed for the first time to generate mutations in Streptomyces fungicidicus. Initial screening detected 44 positive mutants with larger inhibition zone, which were subsequently tested based on flask fermentation. Finally, 20 mutants showed 20% increase in enduracidin production, when compared with the original strain. Among them, enduracidin production by the three mutants (M13, M30, and M34) was significantly higher than that by the original strain. In particular, mutant M30 exhibited highest enduracidin production, which was 114% higher than that obtained with the original strain. Following culture optimization, the maximal enduracidin yield obtained by M30 reached 918.5 mg/L in 10 days, which was 34% higher than that noted in the control. |
| 收录类别 | SCI |
| WOS关键词 | MUTATION ; BEAMS |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000457650400003 |
| 出版者 | WILEY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2558170 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Hu, Wei; Chen, Ji-hong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Lu,Hu, Wei,Li, Wen-jian,et al. Heavy-ion mutagenesis significantly enhances enduracidin production by Streptomyces fungicidicus[J]. ENGINEERING IN LIFE SCIENCES,2019,19(2):112-120. |
| APA | Liu, Lu.,Hu, Wei.,Li, Wen-jian.,Wang, Shu-yang.,Lu, Dong.,...&Chen, Ji-hong.(2019).Heavy-ion mutagenesis significantly enhances enduracidin production by Streptomyces fungicidicus.ENGINEERING IN LIFE SCIENCES,19(2),112-120. |
| MLA | Liu, Lu,et al."Heavy-ion mutagenesis significantly enhances enduracidin production by Streptomyces fungicidicus".ENGINEERING IN LIFE SCIENCES 19.2(2019):112-120. |
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来源：寒区旱区环境与工程研究所
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