Comparative Analysis of Mitochondrial Proteome Reveals the Mechanism of Enhanced Ram Sperm Motility Induced by Carbon Ion Radiation After In Vitro Liquid Storage
文献类型：期刊论文
| 作者 | He, Yuxuan1; Li, Hongyan2,3,4; Zhang, Yong1; Hu, Junjie1; Shen, Yulong1; Feng, Jin1; Zhao, Xingxu1 |
| 刊名 | DOSE-RESPONSE
 |
| 出版日期 | 2019-01-10 |
| 卷号 | 17期号:1页码:13 |
| ISSN号 | 1559-3258 |
| 关键词 | ram sperm mitochondria proteomics carbon ion radiation |
| DOI | 10.1177/1559325818823998 |
| 通讯作者 | Zhao, Xingxu(zhaoxx@gsau.edu.cn) |
| 英文摘要 | The aim of this study was to reveal the mechanism of enhanced ram sperm motility induced by heavy ion radiation (HIR) after in vitro liquid storage. Ram semen was stored for 24 hours at 5 degrees C and then irradiated with 0.1 Gy carbon ion radiation (CIR). In comparison to nonirradiated (NIR) sperm, the motility, viability, and adenosine triphosphate content were all higher in CIR sperm, and the reactive oxygen species levels were lower. Moreover, 87 differential mitochondrial protein spots were detected in 2-dimensional gels between CIR and NIR sperm and were identified as 52 corresponding proteins. In addition, 33 differential proteins were involved in a main pathway network, including COX5B, ERAB/HSD17B10, ETFA, SDHB, and SOD2, which are known to be involved in cell communication, energy production, and antioxidant responses. We used immunoblotting and immunofluorescence to analyze the content and localization of these proteins, respectively, and the levels of these proteins in CIR sperm were lower than those in NIR sperm. An understanding of the molecular function of these proteins could provide further insight into the mechanisms underlying high sperm motility induced by HIR in rams. |
| 收录类别 | SCI |
| WOS关键词 | SPERMATOZOA ; DAMAGE ; CAPACITATION ; IRRADIATION ; PROTEINS ; IDENTIFICATION ; ASSOCIATION ; INFERTILITY ; EXPRESSION ; MUTATION |
| WOS研究方向 | Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging ; Toxicology |
| WOS类目 | Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging ; Toxicology |
| 语种 | 英语 |
| 出版者 | SAGE PUBLICATIONS INC |
| WOS记录号 | WOS:000456388500001 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2558201 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Zhao, Xingxu |
| 作者单位 | 1.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Dept Radiat Med, Lanzhou, Gansu, Peoples R China 3.Chinese Acad Sci, Key Lab Radiat Biol & Med, Lanzhou, Gansu, Peoples R China 4.Key Lab Basic Res Heavy Ion Radiat Applicat Med, Lanzhou, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Yuxuan,Li, Hongyan,Zhang, Yong,et al. Comparative Analysis of Mitochondrial Proteome Reveals the Mechanism of Enhanced Ram Sperm Motility Induced by Carbon Ion Radiation After In Vitro Liquid Storage[J]. DOSE-RESPONSE,2019,17(1):13. |
| APA | He, Yuxuan.,Li, Hongyan.,Zhang, Yong.,Hu, Junjie.,Shen, Yulong.,...&Zhao, Xingxu.(2019).Comparative Analysis of Mitochondrial Proteome Reveals the Mechanism of Enhanced Ram Sperm Motility Induced by Carbon Ion Radiation After In Vitro Liquid Storage.DOSE-RESPONSE,17(1),13. |
| MLA | He, Yuxuan,et al."Comparative Analysis of Mitochondrial Proteome Reveals the Mechanism of Enhanced Ram Sperm Motility Induced by Carbon Ion Radiation After In Vitro Liquid Storage".DOSE-RESPONSE 17.1(2019):13. |
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