Community structure of ammonia-oxidizing bacteria under long-term application of mineral fertilizer and organic manure in a sandy loam soil
文献类型：期刊论文
| 作者 | Chu, Haiyan; Fujii, Takeshi; Morimoto, Sho; Lin, Xiangui; Yagi, Kazuyuki; Hu, Junli; Zhang, Jiabao |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2007 |
| 卷号 | 73期号:2页码:485-491 |
| ISSN号 | 0099-2240 |
| DOI | 10.1128/AEM.01536-06 |
| 通讯作者 | Fujii, Takeshi(ftakeshi@affrc.go.jp) |
| 英文摘要 | The effects of mineral fertilizer (NPK) and organic manure on the community structure of soil ammonia-oxidizing bacteria (AOB) was investigated in a long-term (16-year) fertilizer experiment. The experiment included seven treatments: organic manure, half organic manure N plus half fertilizer N, fertilizer NPK, fertilizer NP, fertilizer NK, fertilizer PK, and the control (without fertilization). N fertilization greatly increased soil nitrification potential, and mineral N fertilizer had a greater impact than organic manure, while N deficiency treatment (PK) had no significant effect. AOB community structure was analyzed by PCR-denaturing gradient gel electrophoresis (PCR-DGGE) of the amoA gene, which encodes the a subunit of ammonia monooxygenase. DGGE profiles showed that the AOB community was more diverse in N-fertilized treatments than in the PK-fertilized treatment or the control, while one dominant band observed in the control could not be detected in any of the fertilized treatments. Phylogenetic analysis showed that the DGGE bands derived from N-fertilized treatments belonged to Nitrosospira cluster 3, indicating that N fertilization resulted in the dominance of Nitrosospira cluster 3 in soil. These results demonstrate that long-term application of N fertilizers could result in increased soil nitrification potential and the AOB community shifts in soil. Our results also showed the different effects of mineral fertilizer N versus organic manure N; the effects of P and K on the soil AOB community; and the importance of balanced fertilization with N, P, and K in promoting nitrification functions in arable soils. |
| 收录类别 | SCI |
| WOS关键词 | 16S RIBOSOMAL-RNA ; GRADIENT GEL-ELECTROPHORESIS ; CLASS PROTEOBACTERIA ; BETA SUBDIVISION ; NITROGEN-FERTILIZER ; MOLECULAR DIVERSITY ; MICROBIAL BIOMASS ; GENE-SEQUENCES ; FOREST SOIL ; POPULATIONS |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000244197400014 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2558660 |
| 专题 | 南京土壤研究所 |
| 通讯作者 | Fujii, Takeshi |
| 作者单位 | 1.Natl Inst Agroenvironm Sci, Tsukuba, Ibaraki 3058604, Japan 2.Chinese Acad Sci, Inst Soil Sci, Nanjing 210008, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chu, Haiyan,Fujii, Takeshi,Morimoto, Sho,et al. Community structure of ammonia-oxidizing bacteria under long-term application of mineral fertilizer and organic manure in a sandy loam soil[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2007,73(2):485-491. |
| APA | Chu, Haiyan.,Fujii, Takeshi.,Morimoto, Sho.,Lin, Xiangui.,Yagi, Kazuyuki.,...&Zhang, Jiabao.(2007).Community structure of ammonia-oxidizing bacteria under long-term application of mineral fertilizer and organic manure in a sandy loam soil.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,73(2),485-491. |
| MLA | Chu, Haiyan,et al."Community structure of ammonia-oxidizing bacteria under long-term application of mineral fertilizer and organic manure in a sandy loam soil".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 73.2(2007):485-491. |
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来源：南京土壤研究所
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