中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Analysis of nifH gene diversity in red soil amended with manure in Jiangxi, south China

文献类型:期刊论文

作者Teng, Qihui1; Sun, Bo2; Fu, Xinrui1; Li, Shunpeng1; Cui, Zhongli1; Cao, Hui1
刊名JOURNAL OF MICROBIOLOGY
出版日期2009-04-01
卷号47期号:2页码:135-141
关键词nifH diversity nitrogen-fixing bacteria red soil manure
ISSN号1225-8873
DOI10.1007/s12275-008-0184-1
通讯作者Cao, Hui(hcao@njau.edu.cn)
英文摘要In order to understand the community structure of diazotrophs in red soil and effects of organic manure Application on the structure, four nifH gene libraries were constructed: the control (CK), low manure (LM), High manure (HM), and high manure adding lime (ML). Totally 150 nifH gene clones were screened and grouped into 21 clusters by RFLP analysis. Existence of dominant patterns was observed in all libraries, which counted for over 96% of clones in library HM and about 56 similar to 72% in other three libraries. The nifH sequences of the dominant patterns in all libraries were most similar to sequences of the cyanobacteria. nifH genes showed high diversity in red soil, dispersing throughout the nifH clades (alpha-, beta-, and gamma-Proteobacteria, Firmicutes, cyanobacteria, Verrucomicrobia, and posited group). Bradyrhizobium and Burkholderia were also important diaxotrophs in low fertility soil samples. Low manure treatment increased the Diversity of nifH genes compared with CK and high manure treatments. Manure and lime treatment led to obvious community succession. Total N to available P ratio, total carbon, and K concentrations were the main factors affecting the diversity of diazotrophs in red soil.
收录类别SCI
WOS关键词NITROGEN-FIXING BACTERIA ; ENVIRONMENTAL-FACTORS ; CYANOBACTERIAL DIVERSITY ; N-2 FIXATION ; RICE FIELDS ; ECOSYSTEMS ; FERTILIZATION ; BIODIVERSITY ; INOCULATION ; COMMUNITIES
WOS研究方向Microbiology
WOS类目Microbiology
语种英语
WOS记录号WOS:000265687300001
出版者MICROBIOLOGICAL SOCIETY KOREA
URI标识http://www.irgrid.ac.cn/handle/1471x/2558753
专题南京土壤研究所
通讯作者Cao, Hui
作者单位1.Nanjing Agr Univ, MOA, Key Lab Microbiol Engn Agr Environm, Nanjing 210095, Peoples R China
2.Chinese Acad Sci, Inst Soil Sci, Nanjing 210008, Peoples R China
推荐引用方式
GB/T 7714
Teng, Qihui,Sun, Bo,Fu, Xinrui,et al. Analysis of nifH gene diversity in red soil amended with manure in Jiangxi, south China[J]. JOURNAL OF MICROBIOLOGY,2009,47(2):135-141.
APA Teng, Qihui,Sun, Bo,Fu, Xinrui,Li, Shunpeng,Cui, Zhongli,&Cao, Hui.(2009).Analysis of nifH gene diversity in red soil amended with manure in Jiangxi, south China.JOURNAL OF MICROBIOLOGY,47(2),135-141.
MLA Teng, Qihui,et al."Analysis of nifH gene diversity in red soil amended with manure in Jiangxi, south China".JOURNAL OF MICROBIOLOGY 47.2(2009):135-141.

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来源:南京土壤研究所

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