A gyrB-targeted PCR for Rapid Identification of Salmonella
文献类型：期刊论文
| 作者 | Ye, Xuhong2,3,4; Wang, Yiming2,3; Lin, Xiangui1,2,3 |
| 刊名 | CURRENT MICROBIOLOGY
 |
| 出版日期 | 2011-11-01 |
| 卷号 | 63期号:5页码:477-483 |
| ISSN号 | 0343-8651 |
| DOI | 10.1007/s00284-011-0007-1 |
| 通讯作者 | Lin, Xiangui(xglin@issas.ac.cn) |
| 英文摘要 | Salmonella causes the majority of infections in humans and homeothermic animals. This article describes a specific polymerase chain reaction (PCR) method developed for a rapid identification of Salmonella. A gyrB-targeted species-specific primer pair, S-P-for (5'-GGT GGT TTC CGT AAA AGT A-3') and S-P-rev (5'-GAA TCG CCT GGT TCT TGC-3'), was successfully designed. PCR with all the Salmonella strains produced a 366- bp DNA fragment that was absent from all the non-Salmonella strains tested. The detection limit of the PCR was 0.01 ng with genomic DNA or 3.2 cells per assay. Good specificity was also demonstrated by fecal samples, from which only the gyrB gene of Salmonella was amplified. Using the culture-PCR method, 27 isolates on Salmonella-Shigella (SS) medium were rapidly identified as Salmonella, which was confirmed by the sequencing of the gyrB gene. |
| 收录类别 | SCI |
| WOS关键词 | POLYMERASE-CHAIN-REACTION ; ENTERITIDIS ; SEQUENCES ; GENE ; FOOD ; MICROORGANISMS ; HYBRIDIZATION ; PATHOGENS ; PROBE |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000295164200012 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2558890 |
| 专题 | 南京土壤研究所 |
| 通讯作者 | Lin, Xiangui |
| 作者单位 | 1.Chinese Acad Sci, Inst Soil Sci, Dept Biol & Biochem, Nanjing 210008, Peoples R China 2.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China 3.Hongkong Baptist Univ, Joint Open Lab Soil & Environm, Nanjing 210008, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Xuhong,Wang, Yiming,Lin, Xiangui. A gyrB-targeted PCR for Rapid Identification of Salmonella[J]. CURRENT MICROBIOLOGY,2011,63(5):477-483. |
| APA | Ye, Xuhong,Wang, Yiming,&Lin, Xiangui.(2011).A gyrB-targeted PCR for Rapid Identification of Salmonella.CURRENT MICROBIOLOGY,63(5),477-483. |
| MLA | Ye, Xuhong,et al."A gyrB-targeted PCR for Rapid Identification of Salmonella".CURRENT MICROBIOLOGY 63.5(2011):477-483. |
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来源：南京土壤研究所
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