STUDY OF IN VITRO INTERACTION BETWEEN TETRABROMOBISPHENOL A AND BOVINE SERUM ALBUMIN BY FLUORESCENCE SPECTROSCOPY
文献类型：期刊论文
| 作者 | Wu, Yingxin1,2; Qian, Yan1; Cui, Hao1; Lai, Xiaomin1; Xie, Xianchuan1,3; Wang, Xiaorong1 |
| 刊名 | ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
 |
| 出版日期 | 2011-12-01 |
| 卷号 | 30期号:12页码:2697-2700 |
| 关键词 | Tetrabromobisphenol A Bovine serum albumin Fluorescence spectroscopy |
| ISSN号 | 0730-7268 |
| DOI | 10.1002/etc.676 |
| 通讯作者 | Xie, Xianchuan(xchxie@yahoo.com.cn) |
| 英文摘要 | The interaction between tetrabromobisphenol A (TBBPA) and bovine serum albumin (BSA) in simulated physiological conditions (pH 7.4) was investigated by fluorescence spectroscopy. The results revealed that TBBPA caused the fluorescence quenching of BSA through a static quenching procedure. The binding constants (K) of TBBPA with BSA at 277, 298, and 310K were obtained as 4.75 x 10(5) L/mol, 5.63 x 10(5) L/mol, and 6.66 x 10(5) L/mol, respectively. There may be two binding sites of TBBPA on BSA. The enthalpy change (Delta H), free energy change (Delta G), and entropy change (Delta S) of thermodynamic parameters indicated that the interaction between TBBPA and BSA was driven mainly by hydrophobic and electrostatic forces. Synchronous fluorescence spectra showed TBBPA binding slightly changed the conformation of BSA by decreasing its polarity and increasing its hydrophobicity. The results of the present study may provide valuable information for studying the distribution and toxicity mechanisms of TBBPA in vivo. Environ. Toxicol. Chem. 2011;30:2697-2700. (C) 2011 SETAC |
| 收录类别 | SCI |
| WOS关键词 | BROMINATED FLAME RETARDANTS ; POLYBROMINATED DIPHENYL ETHERS ; BINDING ; TOXICOKINETICS ; EXPOSURE |
| WOS研究方向 | Environmental Sciences & Ecology ; Toxicology |
| WOS类目 | Environmental Sciences ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000297413100008 |
| 出版者 | WILEY-BLACKWELL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2558891 |
| 专题 | 南京土壤研究所 |
| 通讯作者 | Xie, Xianchuan |
| 作者单位 | 1.Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210008, Peoples R China 2.Sun Yat Sen Univ, Sch Environm Sci & Engn, Guangzhou 510275, Guangdong, Peoples R China 3.Chinese Acad Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Yingxin,Qian, Yan,Cui, Hao,et al. STUDY OF IN VITRO INTERACTION BETWEEN TETRABROMOBISPHENOL A AND BOVINE SERUM ALBUMIN BY FLUORESCENCE SPECTROSCOPY[J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,2011,30(12):2697-2700. |
| APA | Wu, Yingxin,Qian, Yan,Cui, Hao,Lai, Xiaomin,Xie, Xianchuan,&Wang, Xiaorong.(2011).STUDY OF IN VITRO INTERACTION BETWEEN TETRABROMOBISPHENOL A AND BOVINE SERUM ALBUMIN BY FLUORESCENCE SPECTROSCOPY.ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,30(12),2697-2700. |
| MLA | Wu, Yingxin,et al."STUDY OF IN VITRO INTERACTION BETWEEN TETRABROMOBISPHENOL A AND BOVINE SERUM ALBUMIN BY FLUORESCENCE SPECTROSCOPY".ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 30.12(2011):2697-2700. |
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来源：南京土壤研究所
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