Active Autotrophic Ammonia-Oxidizing Bacteria in Biofilm Enrichments from Simulated Creek Ecosystems at Two Ammonium Concentrations Respond to Temperature Manipulation
文献类型：期刊论文
| 作者 | Avrahami, Sharon1,2; Jia, Zhongjun3,4; Neufeld, Josh D.5,6; Murrell, J. Colin6; Conrad, Ralf3; Kuesel, Kirsten1 |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2011-10-01 |
| 卷号 | 77期号:20页码:7329-7338 |
| ISSN号 | 0099-2240 |
| DOI | 10.1128/AEM.05864-11 |
| 通讯作者 | Kuesel, Kirsten(Kirsten.Kuesel@uni-jena.de) |
| 英文摘要 | The first step of nitrification, the oxidation of ammonia to nitrite, is important for reducing eutrophication in freshwater environments when coupled with anammox (anaerobic ammonium oxidation) or denitrification. We analyzed active formerly biofilm-associated aerobic ammonia-oxidizing communities originating from Ammerbach (AS) and Leutra South (LS) stream water (683 +/- 550 [ mean +/- standard deviation] and 16 +/- 7 mu M NH(4)(+), respectively) that were developed in a flow-channel experiment and incubated under three temperature regimens. By stable-isotope probing using (13)CO(2), we found that members of the Bacteria and not Archaea were the functionally dominant autotrophic ammonia oxidizers at all temperatures under relatively high ammonium loads. The copy numbers of bacterial amoA genes in (13)C-labeled DNA were lower at 30 degrees C than at 13 degrees C in both stream enrichment cultures. However, the community composition of the ammonia-oxidizing bacteria (AOB) in the 13C-labeled DNA responded differently to temperature manipulation at two ammonium concentrations. In LS enrichments incubated at the in situ temperature (13 degrees C), Nitrosomonas oligotropha-like sequences were retrieved with sequences from Nitrosospira AmoA cluster 4, while the proportion of Nitrosospira sequences increased at higher temperatures. In AS enrichments incubated at 13 degrees C and 20 degrees C, AmoA cluster 4 sequences were dominant; Nitrosomonas nitrosa-like sequences dominated at 30 degrees C. Biofilm-associated AOB communities were affected differentially by temperature at two relatively high ammonium concentrations, implicating them in a potential role in governing contaminated freshwater AOB distributions. |
| 收录类别 | SCI |
| WOS关键词 | GRADIENT GEL-ELECTROPHORESIS ; COMMUNITY STRUCTURE ; WASTE-WATER ; MESOPHILIC CRENARCHAEOTA ; NITROGEN LIMITATION ; NITRIFYING BACTERIA ; RELATIVE ABUNDANCE ; HEADWATER STREAMS ; TREATMENT PLANTS ; SOIL MICROCOSMS |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000295836700032 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2558907 |
| 专题 | 南京土壤研究所 |
| 通讯作者 | Kuesel, Kirsten |
| 作者单位 | 1.Univ Jena, Inst Ecol Limnol Aquat Geomicrobiol, D-07743 Jena, Germany 2.Technion Israel Inst Technol, Dept Environm Water & Soil Engn, IL-32000 Haifa, Israel 3.Max Planck Inst Terr Microbiol, D-35043 Marburg, Germany 4.Chinese Acad Sci, State Key Lab Soil & Sustainable Agr, Inst Soil Sci, Nanjing 21008, Jiangsu Provinc, Peoples R China 5.Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada 6.Univ Warwick, Sch Life Sci, Coventry CV4 7AL, W Midlands, England |
| 推荐引用方式 GB/T 7714 | Avrahami, Sharon,Jia, Zhongjun,Neufeld, Josh D.,et al. Active Autotrophic Ammonia-Oxidizing Bacteria in Biofilm Enrichments from Simulated Creek Ecosystems at Two Ammonium Concentrations Respond to Temperature Manipulation[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2011,77(20):7329-7338. |
| APA | Avrahami, Sharon,Jia, Zhongjun,Neufeld, Josh D.,Murrell, J. Colin,Conrad, Ralf,&Kuesel, Kirsten.(2011).Active Autotrophic Ammonia-Oxidizing Bacteria in Biofilm Enrichments from Simulated Creek Ecosystems at Two Ammonium Concentrations Respond to Temperature Manipulation.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,77(20),7329-7338. |
| MLA | Avrahami, Sharon,et al."Active Autotrophic Ammonia-Oxidizing Bacteria in Biofilm Enrichments from Simulated Creek Ecosystems at Two Ammonium Concentrations Respond to Temperature Manipulation".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 77.20(2011):7329-7338. |
入库方式： iSwitch采集
来源：南京土壤研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。