A phototrophy-driven microbial food web in a rice soil
文献类型：期刊论文
| 作者 | Feng, Youzhi1; Lin, Xiangui1; Zhu, Jianguo1; Jia, Zhongjun2 |
| 刊名 | JOURNAL OF SOILS AND SEDIMENTS
 |
| 出版日期 | 2011-02-01 |
| 卷号 | 11期号:2页码:301-311 |
| 关键词 | DNA-based stable isotope probing Formate Microbial food web Phototrophy |
| ISSN号 | 1439-0108 |
| DOI | 10.1007/s11368-010-0303-6 |
| 通讯作者 | Lin, Xiangui(xglin@issas.ac.cn) |
| 英文摘要 | Light is a major driver of primary productivity in most ecosystems on Earth. Phototrophic microorganisms harvest light to synthesize organic biomass for sustaining the global energy and carbon flow. However, the bottom-up model of phototrophic microorganisms as primary production and food source for higher trophic levels remains unclear in the terrestrial environment. Rice soil microcosms treated with different carbon sources (C-13-formate, C-12-formate, or a control without formate) were incubated for 21 days under illumination. For each microcosm, genomic DNA were extracted and fractionated by isopycnic ultracentrifugation. Subsequently, the analyses were conducted on these samples with real-time quantitative PCR, PCR-denaturant gradient gel electrophoresis fingerprinting, and sequencing analysis of bacterial 16S rRNA, eukaryotic 18S rRNA, and photosynthetic functional genes. Our analysis indicated that formate carbon was assimilated by a subset of bacteria and eukaryotes. Based on molecular fingerprinting and sequencing analysis, the primary producers were determined to be the microorganisms affiliated with purple phototrophic bacteria, cyanobacteria, and algae. The detection of protozoa and fungi-like 18S rRNA gene sequences in the C-13-enriched DNA fractions suggested that these organisms acted as consumers. They fed on nutrients derived from labeled phototrophic microorganisms. Molecular evidence suggests that in the light-driven microbial food web, the carbon flow from formate is initiated by phototrophic primary and secondary producers. Their biomasses subsequently sustain the growth of consumers in the rice soil. |
| 收录类别 | SCI |
| WOS关键词 | EUKARYOTIC COMMUNITIES ; BACTERIAL COMMUNITY ; PADDY SOIL ; PHYLOGENETIC PROFILE ; PURPLE BACTERIA ; DGGE ANALYSIS ; PCR-DGGE ; FIELD ; SUCCESSION ; IMPACT |
| WOS研究方向 | Geology ; Agriculture |
| WOS类目 | Geosciences, Multidisciplinary ; Soil Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000286435100010 |
| 出版者 | SPRINGER HEIDELBERG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2558937 |
| 专题 | 南京土壤研究所 |
| 通讯作者 | Lin, Xiangui |
| 作者单位 | 1.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Jiangsu Prov, Peoples R China 2.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu Prov, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Youzhi,Lin, Xiangui,Zhu, Jianguo,et al. A phototrophy-driven microbial food web in a rice soil[J]. JOURNAL OF SOILS AND SEDIMENTS,2011,11(2):301-311. |
| APA | Feng, Youzhi,Lin, Xiangui,Zhu, Jianguo,&Jia, Zhongjun.(2011).A phototrophy-driven microbial food web in a rice soil.JOURNAL OF SOILS AND SEDIMENTS,11(2),301-311. |
| MLA | Feng, Youzhi,et al."A phototrophy-driven microbial food web in a rice soil".JOURNAL OF SOILS AND SEDIMENTS 11.2(2011):301-311. |
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来源：南京土壤研究所
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