Oxidation of polycyclic aromatic hydrocarbons by the bacterial laccase CueO from E. coli
文献类型：期刊论文
| 作者 | Zeng, Jun2,3,4; Lin, Xiangui1,2,3; Zhang, Jing2,3; Li, Xuanzhen2,3,4; Wong, Ming Hung3,5 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2011-03-01 |
| 卷号 | 89期号:6页码:1841-1849 |
| 关键词 | Anthracene Bacterial laccase Benzo[a]pyrene Biotransformation CueO Polycyclic aromatic hydrocarbons (PAHs) |
| ISSN号 | 0175-7598 |
| DOI | 10.1007/s00253-010-3009-1 |
| 通讯作者 | Lin, Xiangui(xglin@issas.ac.cn) |
| 英文摘要 | Laccases produced by white rot fungi are capable of rapidly oxidizing benzo[a]pyrene. We hypothesize that the polycyclic aromatic hydrocarbon (PAH)-degrading bacteria producing laccase can enhance the degree of benzo[a]pyrene mineralization. However, fungal laccases are glycoproteins which cannot be glycosylated in bacteria, and there is no evidence to show that bacterial laccases can oxidize benzo[a]pyrene. In this study, the in vitro oxidation of PAHs by crude preparations of the bacterial laccase, CueO, from Escherichia coli was investigated. The results revealed that the crude CueO catalyzed the oxidation of anthracene and benzo[a]pyrene in the same way as the fungal laccase from Trametes versicolor, but showed specific characteristics such as thermostability and copper dependence. In the presence of 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), high amounts of anthracene and benzo[a]pyrene, 80% and 97%, respectively, were transformed under optimal conditions of 60 degrees C, pH 5, and 5 mmol l(-1) CuCl(2) after a 24-h incubation period. Other PAHs including fluorene, acenaphthylene, phenanthrene, and benzo[a]anthracene were also oxidized by the crude CueO. These findings indicated the potential application of prokaryotic laccases in enhancing the mineralization of benzo[a]pyrene by PAH-degrading bacteria. |
| 收录类别 | SCI |
| WOS关键词 | ASCOMYCETE MELANOCARPUS-ALBOMYCES ; FUNGUS PLEUROTUS-OSTREATUS ; MULTICOPPER OXIDASE CUEO ; ESCHERICHIA-COLI ; TRAMETES-VERSICOLOR ; BACILLUS-SUBTILIS ; ELECTRON-TRANSFER ; CRYSTAL-STRUCTURE ; ENDOSPORE COAT ; PURIFICATION |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000288333800018 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2558960 |
| 专题 | 南京土壤研究所 |
| 通讯作者 | Lin, Xiangui |
| 作者单位 | 1.Chinese Acad Sci, Inst Soil Sci, Dept Biol & Biochem, Nanjing 210008, Peoples R China 2.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China 3.Hongkong Baptist Univ, Joint Open Lab Soil & Environm, Nanjing 210008, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 5.Hong Kong Baptist Univ, Croucher Inst Environm Sci, Dept Biol, Kowloon Tong, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zeng, Jun,Lin, Xiangui,Zhang, Jing,et al. Oxidation of polycyclic aromatic hydrocarbons by the bacterial laccase CueO from E. coli[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2011,89(6):1841-1849. |
| APA | Zeng, Jun,Lin, Xiangui,Zhang, Jing,Li, Xuanzhen,&Wong, Ming Hung.(2011).Oxidation of polycyclic aromatic hydrocarbons by the bacterial laccase CueO from E. coli.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,89(6),1841-1849. |
| MLA | Zeng, Jun,et al."Oxidation of polycyclic aromatic hydrocarbons by the bacterial laccase CueO from E. coli".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 89.6(2011):1841-1849. |
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来源：南京土壤研究所
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