Cadmium Uptake, Translocation, and Tolerance in AHA1OX Arabidopsis thaliana
文献类型：期刊论文
| 作者 | Hou, Lingyan1,2; Shi, Weiming2; Wei, Wenhui3; Shen, Hong1 |
| 刊名 | BIOLOGICAL TRACE ELEMENT RESEARCH
 |
| 出版日期 | 2011-02-01 |
| 卷号 | 139期号:2页码:228-240 |
| 关键词 | Arabidopsis Cadmium treatment Citrate secretion Plasma membrane H(+)-ATPase Root-to-shoot Cd translocation |
| ISSN号 | 0163-4984 |
| DOI | 10.1007/s12011-010-8657-6 |
| 通讯作者 | Shen, Hong(hshen@scau.edu.cn) |
| 英文摘要 | Information on cadmium (Cd) uptake and transport is essential to understand better the physiology of Cd tolerance in plants. In this study, Cd uptake, translocation, and tolerance were investigated in AHA1 (Arabidopsis plasma membrane H(+)-ATPase gene) overexpressed plants. Exposed to 10 A mu M CdCl(2), AHA1OX showed a higher root elongation, accumulated more Cd, and maintained better integrity of nucleus membrane of root tips in comparison to the control plant (WT), suggesting that AHA1OX was more Cd tolerant than WT. To investigate Cd tolerance mechanism of AHA1OX plants, we measured the activity of plasma membrane H(+)-ATPase and the secretion of citrate. Results indicated that treatment with 10 A mu M of Cd stimulated the activity of plasma membrane H(+)-ATPase and the secretion of citrate, while 30 A mu M of Cd inhibited them. AHA1OX had higher activity of H(+)-ATPase and secretion of citrate than WT. Addition of citrate enhanced root-to-shoot translocation of Cd significantly. A higher root-to-shoot Cd translocation was observed in AHA1OX than in WT plants. Treatment with low temperature or metabolic inhibitor (carbonyl cyanide m-chlorophenylhydrazone) inhibited Cd uptake and translocation. The study of Cd forms using sequential extraction indicated that Cd was mainly present as a protein-bound form, and AHA1OX had more water-soluble Cd than WT. Taken together, our results suggested that the Cd tolerance of AHA1OX was associated with its root-to-shoot Cd translocation and secretion of citrate, which converts Cd(2+) into less toxic and more easily transportable forms in plant cells. |
| 收录类别 | SCI |
| WOS关键词 | MEMBRANE H+-ATPASE ; HYPERACCUMULATOR THLASPI-CAERULESCENS ; METAL HYPERACCUMULATION ; CD TRANSLOCATION ; ORGANIC-ACIDS ; CITRIC-ACID ; PLANTS ; ACCUMULATION ; TRANSPORT ; HALLERI |
| WOS研究方向 | Biochemistry & Molecular Biology ; Endocrinology & Metabolism |
| WOS类目 | Biochemistry & Molecular Biology ; Endocrinology & Metabolism |
| 语种 | 英语 |
| WOS记录号 | WOS:000286198100012 |
| 出版者 | HUMANA PRESS INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2559050 |
| 专题 | 南京土壤研究所 |
| 通讯作者 | Shen, Hong |
| 作者单位 | 1.S China Agr Univ, Coll Resources & Environm, Guangzhou 510642, Guangdong, Peoples R China 2.Chinese Acad Sci, Inst Soil Sci, Nanjing 210008, Peoples R China 3.Minist Agr, Key Lab Oil Crop Biol, Wuhan 430062, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou, Lingyan,Shi, Weiming,Wei, Wenhui,et al. Cadmium Uptake, Translocation, and Tolerance in AHA1OX Arabidopsis thaliana[J]. BIOLOGICAL TRACE ELEMENT RESEARCH,2011,139(2):228-240. |
| APA | Hou, Lingyan,Shi, Weiming,Wei, Wenhui,&Shen, Hong.(2011).Cadmium Uptake, Translocation, and Tolerance in AHA1OX Arabidopsis thaliana.BIOLOGICAL TRACE ELEMENT RESEARCH,139(2),228-240. |
| MLA | Hou, Lingyan,et al."Cadmium Uptake, Translocation, and Tolerance in AHA1OX Arabidopsis thaliana".BIOLOGICAL TRACE ELEMENT RESEARCH 139.2(2011):228-240. |
入库方式： iSwitch采集
来源：南京土壤研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。