Heat Shock Protein 40 (HSP40) in Pacific White Shrimp (Litopenaeus vannamei): Molecular Cloning, Tissue Distribution and Ontogeny, Response to Temperature, Acidity/Alkalinity and Salinity Stresses, and Potential Role in Ovarian Development
文献类型：期刊论文
| 作者 | Ren, Chunhua4; Chen, Ting4; Lin, Tiehao3; Li, Hongmei; Lu, Ting5; Li, Jiaxi6; Huang, Wen4; Sun, Hongyan5; Jiang, Xiao; Zhang, Jiquan1 |
| 刊名 | FRONTIERS IN PHYSIOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 9页码:1784 |
| 关键词 | heat shock protein HSP40 tissue distribution and ontogeny environmental stress ovarian development crustacean |
| ISSN号 | 1664-042X |
| 英文摘要 | Heat shock proteins (HSPs), a family of conserved proteins that are produced by cells in response to stresses, are known as molecular chaperones with a range of housekeeping and cellular protective functions. The 40 kD heat shock protein (HSP40) is a co-chaperone for HSP70 in the regulation of ATP hydrolysis. Unlike its well-documented cofactor HSP70, little is currently known regarding the biological functions of HSP40 in crustacean species such as penaeid shrimp. In the present study, the cDNA encoding HSP40 (Lv-HSP40) was identified from the Pacific white shrimp Litopenaeus vannamei, a highly significant commercial culture species. The structural organization indicates that Lv-HSP40 belongs to the type-I HSP40s. The muscle, gill, and hepatopancreas are the main sites of Lv-HSP40 transcript expression. Within these tissues, Lv-HSP40 mRNA were predominantly exhibited in the myocytes, epithelial cells and hepatopancreatic cells, respectively. Under acute thermal stress in the culture environment, Lv-HSP40 transcript levels are significantly induced in these three tissues, while low pH stress only upregulates Lv-HSP40 mRNA in the hepatopancreas and gill. During ontogenesis, Lv-HSP40 transcript levels are high at early embryonic stages and drop sharply at late embryonic and early larval stages. The ovary is another major organ of Lv-HSP40 mRNA expression in female shrimp, and Lv-HSP40 transcripts were mainly presented in the follicle cells but only weekly detected in the oocytes. Ovarian Lv-HSP40 mRNA levels increase continuously during gonadal development. Silencing of the Lv-HSP40 gene by RNA interference may effectively delay ovarian maturation after unilateral eyestalk ablation. The roles of Lv-HSP40 in ovarian development are speculated to be independent of its cofactor HSP70, and the vitellogenesis factor vitellogenin (Vg) and vitellogenin receptor (VgR). Our study, as a whole, provides new insights into the roles of HSP40 in multiple physiological processes in L. vannamei: (1) HSP40 is a responding factor during stressful conditions; and (2) HSP40 participates in embryonic and ovarian development. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17592]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Foshan Univ, Foshan, Peoples R China 2.Hebei Univ, Coll Life Sci, Baoding, Peoples R China 3.South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China 4.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou, Guangdong, Peoples R China 5.Guangdong Inst Drug Control, Guangzhou, Guangdong, Peoples R China 6.South China Agr Univ, Coll Marine Sci, Guangzhou, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ren, Chunhua,Chen, Ting,Lin, Tiehao,et al. Heat Shock Protein 40 (HSP40) in Pacific White Shrimp (Litopenaeus vannamei): Molecular Cloning, Tissue Distribution and Ontogeny, Response to Temperature, Acidity/Alkalinity and Salinity Stresses, and Potential Role in Ovarian Development[J]. FRONTIERS IN PHYSIOLOGY,2018,9:1784. |
| APA | Ren, Chunhua.,Chen, Ting.,Lin, Tiehao.,Li, Hongmei.,Lu, Ting.,...&Luo, Peng.(2018).Heat Shock Protein 40 (HSP40) in Pacific White Shrimp (Litopenaeus vannamei): Molecular Cloning, Tissue Distribution and Ontogeny, Response to Temperature, Acidity/Alkalinity and Salinity Stresses, and Potential Role in Ovarian Development.FRONTIERS IN PHYSIOLOGY,9,1784. |
| MLA | Ren, Chunhua,et al."Heat Shock Protein 40 (HSP40) in Pacific White Shrimp (Litopenaeus vannamei): Molecular Cloning, Tissue Distribution and Ontogeny, Response to Temperature, Acidity/Alkalinity and Salinity Stresses, and Potential Role in Ovarian Development".FRONTIERS IN PHYSIOLOGY 9(2018):1784. |
入库方式： OAI收割
来源：南海海洋研究所
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