Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress
文献类型：期刊论文
| 作者 | Luo, Peng; Huang, Wen4; Li, Hongmei3; Cheng, Chuhang3; Ren, Chunhua4; Chen, Ting4; Jiang, Xiao4; Cheng, Kaimin1; Hu, Chaoqun3,4 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2018 |
| 卷号 | 13期号:12页码:e0207771 |
| ISSN号 | 1932-6203 |
| 英文摘要 | Soil salinization. erodes the farmlands and poses a serious threat to human life, reuse of the saline-alkali lands as cultivated resources becomes increasingly prominent. Pacific white shrimp (Litopenaeus vannamei) is an important farmed aquatic species for the development and utilization of the saline-alkali areas. However, little is known about the adaptation mechanism of this species in terms of high-pH stress. In the present study, a transcriptome analysis on the gill tissues of L. vannamei in response to high-pH stress (pH 9.3 +/- 0.1) was conducted. After analyzing, the cyclic nucleotide gated channel-Ca2+ (CNGC-Ca2+) and patched 1 (Ptc1) were detected as the majority annotated components in the cAMP signaling pathway (KO04024), indicating that the CNGC-Ca2+ and Ptc1 might be the candidate components for transducing and maintaining the high-pH stress signals, respectively. The immunoglobulin superfamily (IgSF), heat shock protein (HSP), glutathione s-transferase (GST), prophenoloxidase/phenoloxidase (proPO/PO), superoxide dismutase (SOD), antilipopolysaccharide factor (ALF) and lipoprotein were discovered as the major transcribed immune factors in response to high-pH stress. To further detect hub regulation-genes, protein-protein interaction (PPI) networks were constructed; the genes/proteins "Polymerase (RNA) II (DNA directed) polypeptide A" (POLR2A), "Histone acetyltransferase p300" (EP300) and "Heat shock 70kDa protein 8 '' (HSPA8) were suggested as the top three hub regulation-genes in response to acute high-pH stress; the genes/proteins "Heat shock 70kDa protein 4 '' (HSPA4), "FBJ murine osteosarcoma viral oncogene homolog" (FOS) and "Nucleoporin 54kDa" (NUP54) were proposed as the top three hub regulation-genes involved in adapting endurance high-pH stress; the protein-interactions of "EP300-HSPA8" and "HSPA4-NUP54" were detected as the most important biological interactions in response to the high-pH stress; and the HSP70 family genes might play essential roles in the adaptation of the high-pH stress environment in L. vannamei. These findings provide the first insight into the molecular and immune basis of L. vannamei in terms of high-pH environments, and the construction of a PPI network might improve our understanding in revealing the hub regulation-genes in response to abiotic stress in shrimp species and might be beneficial for further studies. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17595]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Yuehai Feed Grp Co LTD, Zhanjiang, Peoples R China 3.South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China 4.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, Guangzhou, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, Peng,Huang, Wen,Li, Hongmei,et al. Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress[J]. PLOS ONE,2018,13(12):e0207771. |
| APA | Luo, Peng.,Huang, Wen.,Li, Hongmei.,Cheng, Chuhang.,Ren, Chunhua.,...&Hu, Chaoqun.(2018).Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress.PLOS ONE,13(12),e0207771. |
| MLA | Luo, Peng,et al."Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress".PLOS ONE 13.12(2018):e0207771. |
入库方式： OAI收割
来源：南海海洋研究所
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