Subcellular localisation of lipoproteins of Vibrio vulnificus by the identification of outer membrane vesicles components
文献类型：期刊论文
| 作者 | Chen, Shiyong1,2; Chen, Chang1; Wang, Pan; Lin, Huiyuan; Zhang, Yan-Jiao |
| 刊名 | ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 111期号:11页码:1985 |
| 关键词 | +2 rule Lipoprotein Localisation Sorting Outer membrane vesicles Vibrio vulnificus |
| ISSN号 | 0003-6072 |
| 英文摘要 | Vibrio vulnificus, a Gram-negative halophilic bacterium, is an opportunistic human pathogen that is responsible for the majority of seafood-associated deaths worldwide. Lipoproteins are important components of the bacterial cell envelope and have been shown to be involved in a wide variety of cellular processes. Little is known about the localisation or transport mechanism of lipoproteins in V. vulnificus. To assess the localisation of lipoproteins in V. vulnificus, we tested two established techniques for the rapid separation of membrane-associated proteins: detergent extraction with Sarkosyl and outer membrane vesicles (OMVs) preparation. The results showed that Sarkosyl extraction was not useful for the separation of lipoproteins from the different membranes of V. vulnificus. On the other hand, we confirmed that OMVs produced by V. vulnificus contained lipoproteins from the outer but not the inner membrane. Analysis of the OMVs components confirmed the localisation of several well-known lipoproteins to membranes that were different from expected, based on their predicted functions. Using this technique, we found that Asp at position +2 of mature lipoproteins can function as an inner membrane retention signal in V. vulnificus. Interestingly, the Escherichia coli +2 rule does not apply to the V. vulnificus lipoprotein IlpA (G2D) mutant, as a Ser to Asp mutation at position +2 of IlpA did not affect its outer membrane localisation. Furthermore, an IlpA tether-mRFP chimeric lipoprotein and its G2D mutant also behaved like IlpA. Together, these results suggest that the sorting rule of lipoproteins in V. vulnificus might be different from that in E. coli. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17615]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Qingdao Agr Univ, Marine Sci & Engn Coll, Shandong Engn Res Ctr Aquat Anim Immune Preparat, Qingdao 266109, Shandong, Peoples R China 2.Qingdao Agr Univ, Sch Life Sci, Shandong Prov Key Lab Appl Mycol, Qingdao 266109, Shandong, Peoples R China 3.Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Shiyong,Chen, Chang,Wang, Pan,et al. Subcellular localisation of lipoproteins of Vibrio vulnificus by the identification of outer membrane vesicles components[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2018,111(11):1985, 1997. |
| APA | Chen, Shiyong,Chen, Chang,Wang, Pan,Lin, Huiyuan,&Zhang, Yan-Jiao.(2018).Subcellular localisation of lipoproteins of Vibrio vulnificus by the identification of outer membrane vesicles components.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,111(11),1985. |
| MLA | Chen, Shiyong,et al."Subcellular localisation of lipoproteins of Vibrio vulnificus by the identification of outer membrane vesicles components".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 111.11(2018):1985. |
入库方式： OAI收割
来源：南海海洋研究所
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