Soft-shelled turtle iridovirus enters cells via cholesterol-dependent, clathrin-mediated endocytosis as well as macropinocytosis
文献类型：期刊论文
| 作者 | Huang, Xiaohong; Yu, Yepin; Ni, Songwei2,3; Qin, Qiwei; Huang, Youhua; Wang, Shaowen |
| 刊名 | ARCHIVES OF VIROLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 163期号:11页码:3023 |
| ISSN号 | 0304-8608 |
| 英文摘要 | Ranaviruses are nucleoplasmic large DNA viruses that can cause major economic losses in the aquaculture industry and pose a severe threat to global ecological diversity. The available literature demonstrates that classifiable members of the genus Ranavirus enter cells via multiple and complicated routes. Here, we demonstrated the underlying cellular entry mechanism of soft-shelled turtle iridovirus (STIV) using green fluorescence tagged recombinant virus. Treatment with chlorpromazine, sucrose, ethyl-isopropyl amiloride, chloroquine or bafilomycin A1 all significantly decreased STIV infection, suggesting that STIV uses clathrin-mediated endocytosis and macropinocytosis to enter cells via a pH-dependent pathway. Depletion of cellular cholesterol with methyl-beta-cyclodextrin significantly inhibited STIV entry, but neither filipin III nor nystatin did, suggesting that STIV entry was cholesterol dependent but caveola independent. Treatment with dynasore, genistein, ML-7 or cytochalasin D all significantly inhibited STIV infection, indicating that Rac GTPase and myosin II activity were required for the macropinocytosis-like pathway as well as actin polymerization. Our findings suggest that the molecular events involved in STIV entry are not identical to those of other ranavirus isolates. Our results also extend our understanding of the molecular mechanism of iridovirus entry and pathogenesis. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17616]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China 3.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Xiaohong,Yu, Yepin,Ni, Songwei,et al. Soft-shelled turtle iridovirus enters cells via cholesterol-dependent, clathrin-mediated endocytosis as well as macropinocytosis[J]. ARCHIVES OF VIROLOGY,2018,163(11):3023, 3033. |
| APA | Huang, Xiaohong,Yu, Yepin,Ni, Songwei,Qin, Qiwei,Huang, Youhua,&Wang, Shaowen.(2018).Soft-shelled turtle iridovirus enters cells via cholesterol-dependent, clathrin-mediated endocytosis as well as macropinocytosis.ARCHIVES OF VIROLOGY,163(11),3023. |
| MLA | Huang, Xiaohong,et al."Soft-shelled turtle iridovirus enters cells via cholesterol-dependent, clathrin-mediated endocytosis as well as macropinocytosis".ARCHIVES OF VIROLOGY 163.11(2018):3023. |
入库方式： OAI收割
来源：南海海洋研究所
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