Intra-individual variation and transcribed pseudogenes in the ribosomal ITS1-5.8S-ITS2 rDNA of Paraplagusia japonica (Pleuronectiformes: Cynoglossidae)
文献类型：期刊论文
| 作者 | Shi, Wei1,3; Luo, Hairong1,3; Gong, Li2; Yang, Min1,3 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2019 |
| 卷号 | 513期号:3页码:726 |
| 关键词 | Ribosomal RNA Non-concerted evolution Transcribed pseudogene Expression RT-PCR |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2019.04.064 |
| 英文摘要 | The eukaryotic ribosomal DNA (rDNA) cluster consists of multiple copies of three genes (18S, 5.8S, and 28S rDNA) and two internal transcribed spacers (ITS1 and ITS2). Typically, rDNA is thought to be evolved in a strict concerted evolution pattern, in which show little or even no variations within species, while sequence diversity between species or higher taxa. However, in the present study, we reported a high level of intra-individual polymorphism of ITS1-5.8S-ITS2 rDNA in the genome of Paraplagusia japonica (Pleuronectiformes: Cynoglossidae), suggesting it conformed a non-concerted evolutionary pattern. Three divergent type sequences (Type A, B, and C) differing in length, GC content, secondary structure stability and minimum free energy coexisted in the genome of each studied individual. According to the particular features of ribosomal pseudogenes, Type B and Type C sequences were postulated as pseudogenes. Expression analyses gave clear evidence that both the functional gene and pseudogenes were abundantly transcribed, indicating expression is not an applicable criterion for identifying ribosomal pseudogenes. These findings will enrich useful data for the studies on rDNA evolution in flatfishes, as well as provide fresh insight on the transcribed ribosomal pseudogenes and further consideration of the definition of ribosomal pseudogene. (C) 2019 Elsevier Inc. All rights reserved. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17830]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510000, Guangdong, Peoples R China 2.Zhejiang Ocean Univ, Natl Engn Lab Marine Germplasm Resources Explorat, Zhoushan 316022, Peoples R China 3.Zhejiang Ocean Univ, Marine Sci & Technol Coll, Natl Engn Res Ctr Facilitated Marine Aquaculture, Zhoushan 316022, Peoples R China 4.South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510000, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Wei,Luo, Hairong,Gong, Li,et al. Intra-individual variation and transcribed pseudogenes in the ribosomal ITS1-5.8S-ITS2 rDNA of Paraplagusia japonica (Pleuronectiformes: Cynoglossidae)[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2019,513(3):726, 731. |
| APA | Shi, Wei,Luo, Hairong,Gong, Li,&Yang, Min.(2019).Intra-individual variation and transcribed pseudogenes in the ribosomal ITS1-5.8S-ITS2 rDNA of Paraplagusia japonica (Pleuronectiformes: Cynoglossidae).BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,513(3),726. |
| MLA | Shi, Wei,et al."Intra-individual variation and transcribed pseudogenes in the ribosomal ITS1-5.8S-ITS2 rDNA of Paraplagusia japonica (Pleuronectiformes: Cynoglossidae)".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 513.3(2019):726. |
入库方式： OAI收割
来源：南海海洋研究所
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