Biofilm formation in Pseudoalteromonas lipolytica is related to IS5-like insertions in the capsular polysaccharide operon
文献类型：期刊论文
| 作者 | Zeng, Zhenshun; Wang, Pengxia2; Wang, Weiquan1,2; Wang, Xiaoxue1,2; Tang, Kaihao2; Zhan, Waner1,2 |
| 刊名 | FEMS MICROBIOLOGY ECOLOGY
 |
| 出版日期 | 2019 |
| 卷号 | 95期号:6页码:fiz065 |
| 关键词 | insertion sequence capsular polysaccharide biofilm Pseudoalteromonas |
| ISSN号 | 0168-6496 |
| DOI | 10.1093/femsec/fiz065 |
| 英文摘要 | Bacterial capsular polysaccharides (CPSs) participate in environmental adaptation in diverse bacteria species. However, the role and regulation of CPS production in marine bacteria have remained largely unexplored. We previously reported that both wrinkled and translucent Pseudoalteromonas lipolytica variants with altered polysaccharide production were generated in pellicle biofilm-associated cells. In this study, we observed that translucent variants were generated at a rate of similar to 20% in colony biofilms of P. lipolytica cultured on HSLB agar plates for 12 days. The DNA sequencing results revealed that nearly 90% of these variants had an IS5-like element inserted within the coding or promoter regions of nine genes in the cps operon. In contrast, IS5 insertion into the cps operon was not detected in planktonic cells. Furthermore, we demonstrated that the IS5 insertion event inactivated CPS production, which leads to a translucent colony morphology. The CPS-deficient variants showed an increased ability to form attached biofilms but exhibited reduced resistance to sublethal concentrations of antibiotics. Moreover, deleting the DNA repair gene recA significantly decreased the frequency of occurrence of CPS-deficient variants during biofilm formation. Thus, IS insertion into the cps operon is an important mechanism for the production of genetic variants during biofilm formation of marine bacteria. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17831]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, RNAM Ctr Marine Microbiol, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol,Guangdong, Guangzhou 510301, Guangdong, Peoples R China 2.Guangzhou Univ, Sch Environm Sci & Engn, Key Lab Water Qual & Conservat Pearl River Delta, Minist Educ, Guangzhou 510006, Guangdong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zeng, Zhenshun,Wang, Pengxia,Wang, Weiquan,et al. Biofilm formation in Pseudoalteromonas lipolytica is related to IS5-like insertions in the capsular polysaccharide operon[J]. FEMS MICROBIOLOGY ECOLOGY,2019,95(6):fiz065. |
| APA | Zeng, Zhenshun,Wang, Pengxia,Wang, Weiquan,Wang, Xiaoxue,Tang, Kaihao,&Zhan, Waner.(2019).Biofilm formation in Pseudoalteromonas lipolytica is related to IS5-like insertions in the capsular polysaccharide operon.FEMS MICROBIOLOGY ECOLOGY,95(6),fiz065. |
| MLA | Zeng, Zhenshun,et al."Biofilm formation in Pseudoalteromonas lipolytica is related to IS5-like insertions in the capsular polysaccharide operon".FEMS MICROBIOLOGY ECOLOGY 95.6(2019):fiz065. |
入库方式： OAI收割
来源：南海海洋研究所
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