The first cloned sea cucumber FADD from Holothuria leucospilota: Molecular characterization, inducible expression and involvement of apoptosis
文献类型：期刊论文
| 作者 | Sun, Hongyan3; Chen, Ting2; Ren, Chunhua2; Zhao, Lin; Huang, Wen2; Jiang, Xiao2; Wu, Xiaofen2 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2019 |
| 卷号 | 89页码:548 |
| 关键词 | Fas-associated death domain Holothuria leucospilota Apoptosis Innate immune |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2019.04.030 |
| 英文摘要 | In this study, a sea cucumber Fas-associated death domain (FADD) named HLFADD was first cloned from Holothuria leucospilota. The full-length cDNA of HLFADD is 2137 bp in size, containing a 116-bp 5'-untranslated region (UTR), a 1334-bp 3'-UTR and a 687-bp open reading frame (ORF) encoding a protein of 228 amino acids with a deduced molecular weight of 26.42 kDa. HLFADD protein contains a conserved death effector domain at its N-terminal and a conserved death domain at its C-terminal, structurally similar to its counterparts in vertebrates. The over-expressed HLFADD protein could induce apoptosis in HEK293 cells, suggesting a possible death receptor-mediated apoptosis pathway in echinoderms adapted with FADD. Moreover, HLFADD mRNA is ubiquitously expressed in all examined tissues, with the highest transcript level in the coelomocytes, followed by intestine. In vitro experiments performed in the H. leucospilota coelomocytes, the expression of HLFADD mRNA was significantly up-regulated by lipopolysaccharides (LPS) or polyriboinosinic-polyribocytidylic acid [poly (I:C)] challenge, suggesting that HLFADD might play important roles in the innate immune defense of sea cucumber against the invasion of bacteria and viruses. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17835]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.South China Agr Univ, Coll Marine Sci, Joint Lab Guangdong Prov & Hong Kong Reg Marine B, Guangzhou 510642, Guangdong, Peoples R China 2.Guangdong Pharmaceut Univ, Sch Biosci & Biopharmaceut, Guangdong Prov Key Lab Biotechnol Candidate Drug, Guangzhou 510006, Guangdong, Peoples R China 3.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, Guangzhou 510301, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Hongyan,Chen, Ting,Ren, Chunhua,et al. The first cloned sea cucumber FADD from Holothuria leucospilota: Molecular characterization, inducible expression and involvement of apoptosis[J]. FISH & SHELLFISH IMMUNOLOGY,2019,89:548, 554. |
| APA | Sun, Hongyan.,Chen, Ting.,Ren, Chunhua.,Zhao, Lin.,Huang, Wen.,...&Wu, Xiaofen.(2019).The first cloned sea cucumber FADD from Holothuria leucospilota: Molecular characterization, inducible expression and involvement of apoptosis.FISH & SHELLFISH IMMUNOLOGY,89,548. |
| MLA | Sun, Hongyan,et al."The first cloned sea cucumber FADD from Holothuria leucospilota: Molecular characterization, inducible expression and involvement of apoptosis".FISH & SHELLFISH IMMUNOLOGY 89(2019):548. |
入库方式： OAI收割
来源：南海海洋研究所
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