Characterization of one novel microbial esterase WDEst9 and its use to make l-methyl lactate
文献类型：期刊论文
| 作者 | Zhang, Yun1; Sai, Ke3,4,5; Hu, Yunfeng1; Xu, Shan2; Li, Renqiang2; Sun, Aijun1; Wang, Yilong1 |
| 刊名 | BIOCATALYSIS AND BIOTRANSFORMATION
 |
| 出版日期 | 2019 |
| 卷号 | 37期号:3页码:190 |
| ISSN号 | 1024-2422 |
| 关键词 | Biocatalysis novel esterase kinetic resolution L-methyl lactate opposite enantioselectivity |
| DOI | 10.1080/10242422.2018.1526926 |
| 英文摘要 | Chiral lactic acids and their ester derivatives are crucial building blocks and intermediates for the synthesis of a great variety of valuable functional materials and pharmaceuticals. Before our study, the reports about the enantioselective preparation of pure L-lactic acid and its ester derivatives through direct hydrolysis of racemic substrate were quite rare. Herein, we heterologously expressed and functionally characterized one novel microbial esterase WDEst9 from Dactylosporangium aurantiacum, which exhibited high resistance to diverse metal ions, organic solvents, surfactants, NaCl and KCl. We further utilized WDEst9 as a green biocatalyst in the kinetic resolution of (+/-)-methyl lactate through direct hydrolysis and generated L-methyl lactate with high enantiomeric excess (e.e. >99%) and high yield (>86%) after process optimization. Notably, the enantioselectivity of WDEst9 was opposite than that of two previously reported esterases PHE14 and BSE01701 that can generate D-methyl lactate though kinetic resolution of (+/-)-methyl lactate. Microbial esterase WDEst9 is a promising green biocatalyst in the preparation of valuable chiral chemicals and opens the door for the identification of useful industrial enzymes and biocatalysts from the genus Dactylosporangium. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17848]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China 2.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Materia Med, Guangzhou, Guangdong, Peoples R China 3.Jinan Univ, Dept Biotechnol, Guangzhou, Guangdong, Peoples R China 4.Sun Yat Sen Univ, Canc Ctr, Dept Neurosurg Neurooncol, Guangzhou, Guangdong, Peoples R China 5.Collaborat Innovat Ctr Canc Med, Guangzhou, Guangdong, Peoples R China 6.State Key Lab Oncol South China, Guangzhou, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yun,Sai, Ke,Hu, Yunfeng,et al. Characterization of one novel microbial esterase WDEst9 and its use to make l-methyl lactate[J]. BIOCATALYSIS AND BIOTRANSFORMATION,2019,37(3):190, 200. |
| APA | Zhang, Yun.,Sai, Ke.,Hu, Yunfeng.,Xu, Shan.,Li, Renqiang.,...&Wang, Yilong.(2019).Characterization of one novel microbial esterase WDEst9 and its use to make l-methyl lactate.BIOCATALYSIS AND BIOTRANSFORMATION,37(3),190. |
| MLA | Zhang, Yun,et al."Characterization of one novel microbial esterase WDEst9 and its use to make l-methyl lactate".BIOCATALYSIS AND BIOTRANSFORMATION 37.3(2019):190. |
入库方式： OAI收割
来源：南海海洋研究所
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