Characterization of MtdV as a chorismate lyase essential to A201A biosynthesis and precursor-directed biosynthesis of new analogs
文献类型：期刊论文
| 作者 | Ju, Jianhua2; Li, Qinglian; Huang, Hongbo; Song, Yongxiang; Zhu, Qinghua1 |
| 刊名 | ORGANIC & BIOMOLECULAR CHEMISTRY
 |
| 出版日期 | 2019 |
| 卷号 | 17期号:15页码:3760 |
| ISSN号 | 1477-0520 |
| DOI | 10.1039/c8ob02852d |
| 英文摘要 | The antimicrobial nucleoside antibiotic A201A is produced by the deep-sea derived Marinactinospora thermotolerans SCSIO 00652. Bioinformatics analysis revealed that mtdV, downstream within the A201A biosynthetic gene cluster, encodes a protein with low homology to a group of chorismate pyruvatelyases. To explore the role of mtdV in A201A biosynthesis, mtdV was inactivated and HPLC analysis revealed that the resulting Delta mtdV mutant failed to produce A201A; production was partially restored by adding exogenous 4-hydroxybenzoic acid (4HB) to the fermentation. In vitro biochemical assays showed that MtdV catalyzes the conversion of chorismate into 4HB, thereby firmly demonstrating that MtdV is a chorismate lyase involved in A201A biosynthesis. In addition, supplementation of the Delta mtdV mutant with various 4HB analogs enabled production of seven new A201A analogs. Antimicrobial assays showed that the purified A201A analogs 3'-F-A201A and 3'-Cl-A201A were just as active as A201A against the test strains with MIC values of 1-8 mu g mL(-1). |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17858]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China 2.Dezhou Univ, Key Lab Biotechnol & Biol Resource Utilizat Univ, Dezhou 253023, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 10049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ju, Jianhua,Li, Qinglian,Huang, Hongbo,et al. Characterization of MtdV as a chorismate lyase essential to A201A biosynthesis and precursor-directed biosynthesis of new analogs[J]. ORGANIC & BIOMOLECULAR CHEMISTRY,2019,17(15):3760, 3764. |
| APA | Ju, Jianhua,Li, Qinglian,Huang, Hongbo,Song, Yongxiang,&Zhu, Qinghua.(2019).Characterization of MtdV as a chorismate lyase essential to A201A biosynthesis and precursor-directed biosynthesis of new analogs.ORGANIC & BIOMOLECULAR CHEMISTRY,17(15),3760. |
| MLA | Ju, Jianhua,et al."Characterization of MtdV as a chorismate lyase essential to A201A biosynthesis and precursor-directed biosynthesis of new analogs".ORGANIC & BIOMOLECULAR CHEMISTRY 17.15(2019):3760. |
入库方式： OAI收割
来源：南海海洋研究所
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