Molecular evidences on transport of thiocyanate into rice seedlings and assimilation by( 13)C and( 15)N labelling and gene expression analyses
文献类型：期刊论文
| 作者 | Lin, Yu-Juan; Yu, Xiao-Zhang; Shen, Ping-Ping; Zhang, Qing2; Gupta, Dharmendra K. |
| 刊名 | INTERNATIONAL BIODETERIORATION & BIODEGRADATION
 |
| 出版日期 | 2019 |
| 卷号 | 139页码:11 |
| 关键词 | Cyanase Metabolism PCR Rhodanese Rice Stable isotope Thiocyanate |
| ISSN号 | 0964-8305 |
| DOI | 10.1016/j.ibiod.2019.02.003 |
| 英文摘要 | Thiocyanate (SCN -) can be naturally produced by some plant species during assimilation of endogenous cyanide at very low levels, but higher concentration is detected in the environment from various industrial activities, which poses a potential threat to living organisms. In this study, uptake, transport, subcellular distribution and assimilation of SCN - were investigated using rice seedlings exposed to C-13 and N-15-labbled potassium thiocyanate. Our results showed that rice seedlings had a higher potential for SCN (-) uptake, and more was recovered in roots than shoots. Analysis of subcellular partition revealed that SCN- were mainly deposited in cell wall, and followed by cytosol and organelle fractions. Analysis of stable isotopes in rice tissues showed that the molar ratios of C-13 to N-15 content were inconsistent in roots (0.66) and shoots (0.98). PCR analysis revealed different expression arrangements to 20 selected genes in rice tissues, suggesting that involvements of genes encoding with different enzymes in SCN (-) assimilation are tissue specific. These unique genes should regulate and determine degradation pathway of SCN-. Data from stable isotopes and PCR analysis suggests that "TMT (thiol methylation) pathway", "COS (carbonyl sulfide) pathway" and "CNO (cyanate) pathway" are involved in detoxification of SCN- in rice tissues concurrently at the first-degradation phase; Rice seedlings are also able to degrade both CNO and COS in roots effectively, while innate pools of enzymes cyanase (CYN) and carbonyl sulfide hydrolase (COSase) are non-sufficient for further degrade both intermediates in shoots. Our results presented here provide convincing evidence to clarify transport and detoxification pathways of SCN - in rice seedlings. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17869]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.[Yu, Xiao-Zhang 2.Lin, Yu-Juan 3.Guilin Univ Technol, Coll Environm Sci & Engn, Guangxi Key Lab Theory & Technol Environm Pollut, Guilin 541004, Peoples R China 4.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 5.[Gupta, Dharmendra K.] Leibniz Univ Hannover, IRS, Herrenhauser Str 2, D-30419 Hannover, Germany |
| 推荐引用方式 GB/T 7714 | Lin, Yu-Juan,Yu, Xiao-Zhang,Shen, Ping-Ping,et al. Molecular evidences on transport of thiocyanate into rice seedlings and assimilation by( 13)C and( 15)N labelling and gene expression analyses[J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2019,139:11, 17. |
| APA | Lin, Yu-Juan,Yu, Xiao-Zhang,Shen, Ping-Ping,Zhang, Qing,&Gupta, Dharmendra K..(2019).Molecular evidences on transport of thiocyanate into rice seedlings and assimilation by( 13)C and( 15)N labelling and gene expression analyses.INTERNATIONAL BIODETERIORATION & BIODEGRADATION,139,11. |
| MLA | Lin, Yu-Juan,et al."Molecular evidences on transport of thiocyanate into rice seedlings and assimilation by( 13)C and( 15)N labelling and gene expression analyses".INTERNATIONAL BIODETERIORATION & BIODEGRADATION 139(2019):11. |
入库方式： OAI收割
来源：南海海洋研究所
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