Discovery of Stealthin Derivatives and Implication of the Amidotransferase FlsN3 in the Biosynthesis of Nitrogen-Containing Fluostatins
文献类型：期刊论文
| 作者 | Zhu, Yiguang; Huang, Chunshuai1; Yang, Chunfang; Fang, Zhuangjie1; Zhang, Liping; Zhang, Wenjun; Zhang, Changsheng1 |
| 刊名 | MARINE DRUGS
 |
| 出版日期 | 2019 |
| 卷号 | 17期号:3页码:150 |
| 关键词 | angucyclines N-N bond raceme gene inactivation biosynthesis marine Micromonospora |
| ISSN号 | 1660-3397 |
| DOI | 10.3390/md17030150 |
| 英文摘要 | Diazobenzofluorene-containing atypical angucyclines exhibit promising biological activities. Here we report the inactivation of an amidotransferase-encoding gene flsN3 in Micromonospora rosaria SCSIO N160, a producer of fluostatins. Bioinformatics analysis indicated that FlsN3 was involved in the diazo formation. Chemical investigation of the flsN3-inactivation mutant resulted in the isolation of a variety of angucycline aromatic polyketides, including four racemic aminobenzo[b]fluorenes stealthins D-G (9-12) harboring a stealthin C-like core skeleton with an acetone or butanone-like side chain. Their structures were elucidated on the basis of nuclear magnetic resonance (NMR) spectroscopic data and X-ray diffraction analysis. A plausible mechanism for the formation of stealthins D-G (9-12) was proposed. These results suggested a functional role of FlsN3 in the formation/modification of N-N bond-containing fluostatins. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17880]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Inst South China Sea Ecol & Environm Engn,Guandon, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China 2.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Yiguang,Huang, Chunshuai,Yang, Chunfang,et al. Discovery of Stealthin Derivatives and Implication of the Amidotransferase FlsN3 in the Biosynthesis of Nitrogen-Containing Fluostatins[J]. MARINE DRUGS,2019,17(3):150. |
| APA | Zhu, Yiguang.,Huang, Chunshuai.,Yang, Chunfang.,Fang, Zhuangjie.,Zhang, Liping.,...&Zhang, Changsheng.(2019).Discovery of Stealthin Derivatives and Implication of the Amidotransferase FlsN3 in the Biosynthesis of Nitrogen-Containing Fluostatins.MARINE DRUGS,17(3),150. |
| MLA | Zhu, Yiguang,et al."Discovery of Stealthin Derivatives and Implication of the Amidotransferase FlsN3 in the Biosynthesis of Nitrogen-Containing Fluostatins".MARINE DRUGS 17.3(2019):150. |
入库方式： OAI收割
来源：南海海洋研究所
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