Resistance to oxidative stress by inner membrane protein ElaB is regulated by OxyR and RpoS
文献类型：期刊论文
| 作者 | Guo, Yunxue; Li, Yangmei1; Zhan, Waner1; Wood, Thomas K.; Wang, Xiaoxue1 |
| 刊名 | MICROBIAL BIOTECHNOLOGY
 |
| 出版日期 | 2019 |
| 卷号 | 12期号:2页码:392 |
| ISSN号 | 1751-7915 |
| DOI | 10.1111/1751-7915.13369 |
| 英文摘要 | C-tail anchored inner membrane proteins are a family of proteins that contain a C-terminal transmembrane domain but lack an N-terminal signal sequence for membrane targeting. They are widespread in eukaryotes and prokaryotes and play critical roles in membrane traffic, apoptosis and protein translocation in eukaryotes. Recently, we identified and characterized in Escherichia coli a new C-tail anchored inner membrane, ElaB, which is regulated by the stationary phase sigma factor RpoS. ElaB is important for resistance to oxidative stress but the exact mechanism is unclear. Here, we show that ElaB functions as part of the adaptive oxidative stress response by maintaining membrane integrity. Production of ElaB is induced by oxidative stress at the transcriptional level. Moreover, elaB expression is also regulated by the key regulator OxyR via an OxyR binding site in the promoter of elaB. OxyR induces the expression of elaB in the exponential growth phase, while excess OxyR reduces elaB expression in an RpoS-dependent way in the stationary phase. In addition, deletion of elaB reduced fitness compared to wild-type cells after prolonged incubation. Therefore, we determined how ElaB is regulated under oxidative stress: RpoS and OxyR coordinately control the expression of inner membrane protein ElaB. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17886]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, South China Sea Inst Oceanol, RNAM Ctr Marine Microbiol, CAS Key Lab Trop Marine Bioresources & Ecol,Guang, Guangzhou 510301, Guangdong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Wood, Thomas K.] Penn State Univ, Dept Chem Engn, University Pk, PA 16802 USA 4.Wood, Thomas K.] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA |
| 推荐引用方式 GB/T 7714 | Guo, Yunxue,Li, Yangmei,Zhan, Waner,et al. Resistance to oxidative stress by inner membrane protein ElaB is regulated by OxyR and RpoS[J]. MICROBIAL BIOTECHNOLOGY,2019,12(2):392, 404. |
| APA | Guo, Yunxue,Li, Yangmei,Zhan, Waner,Wood, Thomas K.,&Wang, Xiaoxue.(2019).Resistance to oxidative stress by inner membrane protein ElaB is regulated by OxyR and RpoS.MICROBIAL BIOTECHNOLOGY,12(2),392. |
| MLA | Guo, Yunxue,et al."Resistance to oxidative stress by inner membrane protein ElaB is regulated by OxyR and RpoS".MICROBIAL BIOTECHNOLOGY 12.2(2019):392. |
入库方式： OAI收割
来源：南海海洋研究所
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