Purification and identification of intestinal mucosal cell proliferation-promoting peptides from Crassostrea hongkongensis
文献类型:期刊论文
作者 | Sun, Huili; Pan, Jianyu; Wan, Peng; Chen, Deke; Chen, Hua; Chen, Xin2; Cai, Bingna |
刊名 | EUROPEAN FOOD RESEARCH AND TECHNOLOGY |
出版日期 | 2019 |
卷号 | 245期号:3页码:631 |
ISSN号 | 1438-2377 |
关键词 | Peptides Crassostrea hongkongensis Cell proliferation promotion Cell migration promotion Intestinal epithelia |
DOI | 10.1007/s00217-018-3186-1 |
英文摘要 | Oyster peptides, together with polysaccharides, were found to protect the intestinal mucosal barrier against the harmful effects of 5-FU chemotherapy on rats in our previous study. However, whether oyster (Crassostrea hongkongensis) peptide can promote intestinal epithelia cell proliferation and migration alone is unknown. In this paper, oyster tissue was hydrolyzed using pepsin, trypsin, papain, bromelain, neutrase, flavorzyme, and alcalase protease. Among the hydrolysates that produced by trypsin showed the highest promoting effect on intestinal epithelia cell (IEC-6) proliferation and was fractionated into three molecular weight ultra-filtrates of >10, 10-5, and <5kDa. The 10-5kDa ultra-filtrate showed the highest promoting effect on cell proliferation, at 146.16 +/- 6.56% (0.16mg/ml), and was subjected to further purification. Two oyster peptides (F3-1 and F3-2) were purified from the 5-10kDa ultra-filtrate by SEC and RP-HPLC. Their molecular mass and amino acid sequences were identified as VAPEEHPVLL (MW, 1102.5872Da) and SYELPDGQVITIGNER (MW, 1789.9247Da) by MALDI-TOF-MS/MS. Peptides F3-1 and F3-2 exhibited 150.81 +/- 12.34% (5 mu g/ml) and 151.73 +/- 12.28% (6.25 mu g/ml) promoting effects on IEC-6 proliferation, respectively, and both peptides showed greater promoting effects than the positive control (proglumide) at the same concentration under LPS stimulation culture conditions. This study indicated that oyster peptides could serve as a potential source of a functional food constituent for intestinal epithelium protection. |
源URL | [http://ir.scsio.ac.cn/handle/344004/17891] |
专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
作者单位 | 1.Foshan Univ, Dept Chem & Chem Engn, Foshan 528000, Peoples R China 2.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Meteria Med, Key Lab Trop Marine Bioresources & Ecol, 164 Xingangxi Rd,POB 510301, Guangzhou, Guangdong, Peoples R China |
推荐引用方式 GB/T 7714 | Sun, Huili,Pan, Jianyu,Wan, Peng,et al. Purification and identification of intestinal mucosal cell proliferation-promoting peptides from Crassostrea hongkongensis[J]. EUROPEAN FOOD RESEARCH AND TECHNOLOGY,2019,245(3):631, 642. |
APA | Sun, Huili.,Pan, Jianyu.,Wan, Peng.,Chen, Deke.,Chen, Hua.,...&Cai, Bingna.(2019).Purification and identification of intestinal mucosal cell proliferation-promoting peptides from Crassostrea hongkongensis.EUROPEAN FOOD RESEARCH AND TECHNOLOGY,245(3),631. |
MLA | Sun, Huili,et al."Purification and identification of intestinal mucosal cell proliferation-promoting peptides from Crassostrea hongkongensis".EUROPEAN FOOD RESEARCH AND TECHNOLOGY 245.3(2019):631. |
入库方式: OAI收割
来源:南海海洋研究所
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