中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Purification and identification of intestinal mucosal cell proliferation-promoting peptides from Crassostrea hongkongensis

文献类型:期刊论文

作者Sun, Huili; Pan, Jianyu; Wan, Peng; Chen, Deke; Chen, Hua; Chen, Xin2; Cai, Bingna
刊名EUROPEAN FOOD RESEARCH AND TECHNOLOGY
出版日期2019
卷号245期号:3页码:631
ISSN号1438-2377
关键词Peptides Crassostrea hongkongensis Cell proliferation promotion Cell migration promotion Intestinal epithelia
DOI10.1007/s00217-018-3186-1
英文摘要Oyster peptides, together with polysaccharides, were found to protect the intestinal mucosal barrier against the harmful effects of 5-FU chemotherapy on rats in our previous study. However, whether oyster (Crassostrea hongkongensis) peptide can promote intestinal epithelia cell proliferation and migration alone is unknown. In this paper, oyster tissue was hydrolyzed using pepsin, trypsin, papain, bromelain, neutrase, flavorzyme, and alcalase protease. Among the hydrolysates that produced by trypsin showed the highest promoting effect on intestinal epithelia cell (IEC-6) proliferation and was fractionated into three molecular weight ultra-filtrates of >10, 10-5, and <5kDa. The 10-5kDa ultra-filtrate showed the highest promoting effect on cell proliferation, at 146.16 +/- 6.56% (0.16mg/ml), and was subjected to further purification. Two oyster peptides (F3-1 and F3-2) were purified from the 5-10kDa ultra-filtrate by SEC and RP-HPLC. Their molecular mass and amino acid sequences were identified as VAPEEHPVLL (MW, 1102.5872Da) and SYELPDGQVITIGNER (MW, 1789.9247Da) by MALDI-TOF-MS/MS. Peptides F3-1 and F3-2 exhibited 150.81 +/- 12.34% (5 mu g/ml) and 151.73 +/- 12.28% (6.25 mu g/ml) promoting effects on IEC-6 proliferation, respectively, and both peptides showed greater promoting effects than the positive control (proglumide) at the same concentration under LPS stimulation culture conditions. This study indicated that oyster peptides could serve as a potential source of a functional food constituent for intestinal epithelium protection.
源URL[http://ir.scsio.ac.cn/handle/344004/17891]  
专题南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位1.Foshan Univ, Dept Chem & Chem Engn, Foshan 528000, Peoples R China
2.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Meteria Med, Key Lab Trop Marine Bioresources & Ecol, 164 Xingangxi Rd,POB 510301, Guangzhou, Guangdong, Peoples R China
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Sun, Huili,Pan, Jianyu,Wan, Peng,et al. Purification and identification of intestinal mucosal cell proliferation-promoting peptides from Crassostrea hongkongensis[J]. EUROPEAN FOOD RESEARCH AND TECHNOLOGY,2019,245(3):631, 642.
APA Sun, Huili.,Pan, Jianyu.,Wan, Peng.,Chen, Deke.,Chen, Hua.,...&Cai, Bingna.(2019).Purification and identification of intestinal mucosal cell proliferation-promoting peptides from Crassostrea hongkongensis.EUROPEAN FOOD RESEARCH AND TECHNOLOGY,245(3),631.
MLA Sun, Huili,et al."Purification and identification of intestinal mucosal cell proliferation-promoting peptides from Crassostrea hongkongensis".EUROPEAN FOOD RESEARCH AND TECHNOLOGY 245.3(2019):631.

入库方式: OAI收割

来源:南海海洋研究所

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