Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells
文献类型：期刊论文
| 作者 | Sun, Huili; Cai, Bingna; Wan, Peng; Chen, Hua; Chen, Deke; Chen, Xin1; Pan, Jianyu |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2019 |
| 卷号 | 124页码:246 |
| ISSN号 | 0141-8130 |
| 关键词 | Oyster polysaccharide IEC-6 Oxidative stress |
| DOI | 10.1016/j.ijbiomac.2018.11.154 |
| 英文摘要 | The proliferative activity of oyster polysaccharides in intestine epithelial cells (IEC-6) alleviated 5-fluorouracil-induced intestinal inflammation. In this study, we aimed to measure the ability of oyster polysaccharides to promote IEC-6 cell migration and antioxidant activity and further describe their cytoprotective effect on H2O2-challenged IEC-6 cells. The C30-60% fraction of polysaccharides (CHP2) showed rapid stimulation of IEC-6 cell migration after wounding. Then, CHP2 was fractionated into four fractions, namely, CHP2-1, CHP2-2, CHP2-3 and CHP2-4. The CHP2-3 fraction possessed high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC), in comparison with other fractions. And CHP2-3 was heteropolysaccharide with sulfuric esters, and it was mainly composed of glucose, galactose and arabinose and had an average molecular weight of 41.81 kDa. Pretreatment with CHP2 and CHP2-3 significantly improved the survival rate of H2O2-treated IEC-6 cells, and reduced intracellular reactive oxygen species (ROS) levels. Moreover, CHP2-3 also significantly decreased H2O2-mediated increases in the secretion of interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6), and attenuated nuclear factor-kappa B (NF-kappa B) p65 activation. These results indicate that CHP2-3 may play a vital role in reducing oxidative damage in IEC-6 cells via radical scavenging, decreasing proinflammatory factors secretion, inhibiting the NF-kappa B pathway, and thus, reducing cell apoptosis. (C) 2018 Elsevier B.V. All rights reserved. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17892]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, Guangzhou, Guangdong, Peoples R China 2.Foshan Univ, Sch Environm & Chem Engn, Foshan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Huili,Cai, Bingna,Wan, Peng,et al. Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,124:246, 254. |
| APA | Sun, Huili.,Cai, Bingna.,Wan, Peng.,Chen, Hua.,Chen, Deke.,...&Pan, Jianyu.(2019).Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,124,246. |
| MLA | Sun, Huili,et al."Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 124(2019):246. |
入库方式： OAI收割
来源：南海海洋研究所
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