Induction of Apoptosis in Human Glioma Cells by Fucoxanthin via Triggering of ROS-Mediated Oxidative Damage and Regulation of MAPKs and PI3K-AKT Pathways
文献类型：期刊论文
| 作者 | Fu, Xiao-yan; Fan, Cun-dong; Wang, Ying; Ma, Jin-kui; Hou, Ya-jun; Xiang, Wen-zhou; Cao, Wen-giang; Wu, Hua-lian |
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| 出版日期 | 2019 |
| 卷号 | 67期号:8页码:2212 |
| 关键词 | fucoxanthin glioma cells reactive oxygen species apoptosis oxidative damage |
| ISSN号 | 0021-8561 |
| DOI | 10.1021/acs.jafc.8b07126 |
| 英文摘要 | Fucoxanthin, a natural carotenoid derived from algae, exhibits novel anticancer potential. However, fucoxanthin with high purity is hard to prepare, and the anticancer mechanism remains elusive. In the present study, fucoxanthin with high purity was prepared and purified from the marine microalgae Nitzschia sp. by silica-gel column chromatography (SGCC), and the underlying mechanism against human glioma cells was evaluated. The results showed that fucoxanthin time- and dose-dependently inhibited U251-human-glioma-cell growth by induction of apoptosis (64.4 +/- 4.8, P < 0.01) accompanied by PARP cleavage and caspase activation (244 +/- 14.2, P < 0.01). Mechanically, fucoxanthin time-dependently triggered reactive-oxygen-species (ROS)-mediated DNA damage (100 +/- 7.38, P < 0.01), as evidenced by the phosphorylation activation of Ser1981-ATM, Ser428-ATR, Ser15-p53, and Ser139-histone. Moreover, fucoxanthin treatment also time-dependently caused dysfunction of MAPKs and PI3K-AKT pathways, as demonstrated by the phosphorylation activation of Thr183-JNK, Thr180-p38, and Thr202-ERK and the phosphorylation inactivation of Ser473-AKT. The addition of kinase inhibitors further confirmed the importance of MAPKs and PI3K-AKT pathways in fucoxanthin-induced cell-growth inhibition (32.5 +/- 3.6, P < 0.01). However, ROS inhibition by the antioxidant glutathione (GSH) effectively inhibited fucoxanthin-induced DNA damage, attenuated the dysfunction of MAPKs and PI3K-AKT pathways, and eventually blocked fucoxanthin-induced cytotoxicity (54.3 +/- 5.6, P < 0.05) and cell apoptosis (32.7 +/- 2.5, P < 0.05), indicating that ROS production, an early apoptotic event, is involved in the fucoxanthin-mediated anticancer mechanism. Taken together, these results suggested that fucoxanthin induced U251-human-glioma-cell apoptosis by triggering ROS-mediated oxidative damage and dysfunction of MAPKs and PI3K-AKT pathways, which validated that fucoxanthin may be a candidate for potential applications in cancer chemotherapy and chemoprevention. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17896]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Hou, Ya-jun 2.[Fu, Xiao-yan 3.Wang, Ying 4.Zhuhai Hopegenes Med & Phamaceut Inst, Hengqin New Area, Zhuhai 519000, Guangdong, Peoples R China 5.Taishan Med Univ, Key Lab Cerebral Microcirculat Univ Shandong, Tai An 271000, Shandong, Peoples R China 6.Zhaoqing Univ, Sch Food & Pharmaceut Engn, Zhaoqing 526061, Guangdong, Peoples R China 7.[Wu, Hua-lian 8.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB C, Guangdong Key Lab Marine Mat Med LMMM GD, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Xiao-yan,Fan, Cun-dong,Wang, Ying,et al. Induction of Apoptosis in Human Glioma Cells by Fucoxanthin via Triggering of ROS-Mediated Oxidative Damage and Regulation of MAPKs and PI3K-AKT Pathways[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(8):2212, 2219. |
| APA | Fu, Xiao-yan.,Fan, Cun-dong.,Wang, Ying.,Ma, Jin-kui.,Hou, Ya-jun.,...&Wu, Hua-lian.(2019).Induction of Apoptosis in Human Glioma Cells by Fucoxanthin via Triggering of ROS-Mediated Oxidative Damage and Regulation of MAPKs and PI3K-AKT Pathways.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(8),2212. |
| MLA | Fu, Xiao-yan,et al."Induction of Apoptosis in Human Glioma Cells by Fucoxanthin via Triggering of ROS-Mediated Oxidative Damage and Regulation of MAPKs and PI3K-AKT Pathways".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.8(2019):2212. |
入库方式： OAI收割
来源：南海海洋研究所
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