Na+/H+ exchanger (NHE) in Pacific white shrimp (Litopenaeus vannamei): Molecular cloning, transcriptional response to acidity stress, and physiological roles in pH homeostasis
文献类型：期刊论文
| 作者 | Hu, Chaoqun2; Cheng, Chuhang1; Jiang, Xiao2; Ren, Chunhua2; Li, Hongmei1; Ruan, Yao1; Zhang, Xin1; Huang, Wen2; Chen, Ting2 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2019 |
| 卷号 | 14期号:2页码:e0212887 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0212887 |
| 英文摘要 | Na+/H+ exchangers are the most common membrane proteins involved in the regulation of intracellular pH that concurrently transport Na+ into the cells and H+ out of the cells. In this study, the full-length cDNA of the Na+/H+ exchanger (NHE) from the Pacific white shrimp (Litopenaeus vannamei) was cloned. The LvNHE cDNA is 3167 bp long, contains a 5'-untranslated region (UTR) of 74 bp and a 3'-UTR of 456 bp and an open reading frame (ORF) of 2637 bp, coding for a protein of 878 amino acids with 11 putative transmembrane domains and a long cytoplasmic tail. LvNHE shows high sequence homology with mud crab NHE at the amino acid level. LvNHE mRNA was detected in the hepatopancreas, gill, eyestalk, skin, heart, intestine, muscle, brain and stomach, with the highest abundance in the intestine. In the shrimp intestinal fragment cultures exposed to gradually declining pH medium (from pH 8.0 to pH 6.4), the LvNHE mRNA expression was significantly stimulated, with the highest response when incubated in pH 7.0 medium for 6 h. To investigate the functional roles of LvNHE in pH regulation at the physiological and cellular levels, the LvNHE mRNA expression was silenced by siRNA knockdown. Upon low-pH challenge, the hemolymph pH was significantly reduced in the LvNHE mRNA knockdown shrimp. In addition, knockdown of LvNHE mRNA reduced the recovery capacity of intracellular pH in intestinal fragment cultures after acidification. Altogether, this study demonstrates the role of NHE in shrimp response to low pH stress and provides new insights into the acid/base homeostasis mechanisms of crustaceans. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17897]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, South China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Chaoqun,Cheng, Chuhang,Jiang, Xiao,et al. Na+/H+ exchanger (NHE) in Pacific white shrimp (Litopenaeus vannamei): Molecular cloning, transcriptional response to acidity stress, and physiological roles in pH homeostasis[J]. PLOS ONE,2019,14(2):e0212887. |
| APA | Hu, Chaoqun.,Cheng, Chuhang.,Jiang, Xiao.,Ren, Chunhua.,Li, Hongmei.,...&Chen, Ting.(2019).Na+/H+ exchanger (NHE) in Pacific white shrimp (Litopenaeus vannamei): Molecular cloning, transcriptional response to acidity stress, and physiological roles in pH homeostasis.PLOS ONE,14(2),e0212887. |
| MLA | Hu, Chaoqun,et al."Na+/H+ exchanger (NHE) in Pacific white shrimp (Litopenaeus vannamei): Molecular cloning, transcriptional response to acidity stress, and physiological roles in pH homeostasis".PLOS ONE 14.2(2019):e0212887. |
入库方式： OAI收割
来源：南海海洋研究所
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