Excisionase in Pf filamentous prophage controls lysis-lysogeny decision-making in Pseudomonas aeruginosa
文献类型：期刊论文
| 作者 | Guo, Yunxue; Li, Yangmei2; Liu, Xiaoxiao; Tang, Kaihao; Wang, Pengxia; Zeng, Zhenshun; Wang, Xiaoxue2 |
| 刊名 | MOLECULAR MICROBIOLOGY
 |
| 出版日期 | 2019 |
| 卷号 | 111期号:2页码:495 |
| ISSN号 | 0950-382X |
| DOI | 10.1111/mmi.14170 |
| 英文摘要 | Pf filamentous prophages are prevalent among clinical and environmental Pseudomonas aeruginosa isolates. Pf4 and Pf5 prophages are integrated into the host genomes of PAO1 and PA14, respectively, and play an important role in biofilm development. However, the genetic factors that directly control the lysis-lysogeny switch in Pf prophages remain unclear. Here, we identified and characterized the excisionase genes in Pf4 and Pf5 (named xisF4 and xisF5, respectively). XisF4 and XisF5 represent two major subfamilies of functional excisionases and are commonly found in Pf prophages. While both of them can significantly promote prophage excision, only XisF5 is essential for Pf5 excision. XisF4 activates Pf4 phage replication by upregulating the phage initiator gene (PA0727). In addition, xisF4 and the neighboring phage repressor c gene pf4r are transcribed divergently and their 5 '-untranslated regions overlap. XisF4 and Pf4r not only auto-activate their own expression but also repress each other. Furthermore, two H-NS family proteins, MvaT and MvaU, coordinately repress Pf4 production by directly repressing xisF4. Collectively, we reveal that Pf prophage excisionases cooperate in controlling lysogeny and phage production. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17913]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, Guangzhou 510301, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Yunxue,Li, Yangmei,Liu, Xiaoxiao,et al. Excisionase in Pf filamentous prophage controls lysis-lysogeny decision-making in Pseudomonas aeruginosa[J]. MOLECULAR MICROBIOLOGY,2019,111(2):495, 513. |
| APA | Guo, Yunxue.,Li, Yangmei.,Liu, Xiaoxiao.,Tang, Kaihao.,Wang, Pengxia.,...&Wang, Xiaoxue.(2019).Excisionase in Pf filamentous prophage controls lysis-lysogeny decision-making in Pseudomonas aeruginosa.MOLECULAR MICROBIOLOGY,111(2),495. |
| MLA | Guo, Yunxue,et al."Excisionase in Pf filamentous prophage controls lysis-lysogeny decision-making in Pseudomonas aeruginosa".MOLECULAR MICROBIOLOGY 111.2(2019):495. |
入库方式： OAI收割
来源：南海海洋研究所
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