Pf-Dmrt4, a potential factor in sexual development in the pearl oyster Pinctada fucata
文献类型：期刊论文
| 作者 | Shi Yu; He Maoxian; Wang Qi1 |
| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 36期号:6页码:2337 |
| 关键词 | Pinctada fucata Pf-Dmrt4 gene expression sexual development |
| ISSN号 | 2096-5508 |
| DOI | 10.1007/s00343-019-7177-2 |
| 英文摘要 | The mechanisms of sex determination and sex differentiation in the pearl oyster Pinctada fucata are currently poorly understood. We therefore investigated the roles of orthologs of the Dmrt gene family, key players in male gonad differentiation in mammals, in P. fucata sex differentiation and sexual development. Pf-Dmrt4 exhibits features typical of the Dmrt family, and displays significant homologies to the DMRT4 cluster. Pf-Dmrt4 mRNA expression in the gonads during a gametogenic cycle, measured by quantitative polymerase chain reaction, was maximal in mature individuals. Pf-Dmrt4 expression, demonstrated by in situ hybridization, was localized in the spermatozoa, spermatids, oocytes and vitellogenic oocytes. Knockdown of Pf-Dmrt4 with double-stranded RNA resulted in decreased mRNA expression levels. And Pf-Dmrt4-dsRNA-injected groups showed spawning-stage male gonads, with ruptured follicles and released spermatozoa. Our results enhance the understanding of sex determination and differentiation in P.fucata and suggest that Pf-Dmrt4 could be involved in male gonadal development, and maintenance of male gonadal function. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17933]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 2.Taiyuan Univ Technol, Taiyuan 030024, Shanxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi Yu,He Maoxian,Wang Qi. Pf-Dmrt4, a potential factor in sexual development in the pearl oyster Pinctada fucata[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(6):2337, 2350. |
| APA | Shi Yu,He Maoxian,&Wang Qi.(2018).Pf-Dmrt4, a potential factor in sexual development in the pearl oyster Pinctada fucata.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(6),2337. |
| MLA | Shi Yu,et al."Pf-Dmrt4, a potential factor in sexual development in the pearl oyster Pinctada fucata".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.6(2018):2337. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。