Characterization of a new bradykinin-potentiating peptide (TmF) from Trimeresurus mucrosquamatus
文献类型：期刊论文
| 作者 | Jia, YH ; Li, DS ; Zhu, SW ; Zhang, LY ; Ding, LS ; Wang, WY ; Xiong, YL |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:7页码:619_623 |
| 关键词 | angiotensin-converting enzyme bradykinin bradykinin-potentiating peptide TmF |
| ISSN号 | 0582-9879 |
| 产权排序 | 3 |
| 通讯作者 | Xiong, YL, Chinese Acad Sci, Kunming Inst Zool, Dept Anim Toxicol, Kunming 650223, Peoples R China. |
| 中文摘要 | A novel bradykinin-potentiating peptide (BPP), designated as TmF, has been purified to homogeneity from the venom of Trimeresurus mucrosquamatus by 70% cold methanol extraction, Sephadex G-15 gel filtration and reverse-phase high performance liquid chromatography (RP-HPLC). The amino acid sequence of TmF was determined to be pGlu-Gly-Arg-Pro-Leu-Gly-Pro-Pro-Ile-Pro-Pro (pGlu denotes pyroglutamic acid), which shared high homology with other BPPs. The molecular mass of TmF was 1.1107 kD as determinated by electrospray ionization-mass spectrometry (ESI-MS), which was in accordance with the calculated value of 1.1106 kD. The potentiating "unit" of TmF to bradykinin-induced (BK-induced) contraction on the guinea-pig ileum in vitro was (1.13 +/- 0.3) unit (mg/L), and TmF (5.0 x 10(-4) mg/kg) increased the pressure-lowering-effect of bradykinin (5.0 x 10(-5) mg/kg) with approximate descent value of (14 +/- 2) mmHg. In addition, TmF inhibited the conversion of angiotensin I to angiotensin 11, 2 x 10(-3) mg of TmF caused 50% inhibition (IC50) of angiotensin-converting enzyme (ACE) hydrolyzing activity to bradykinin. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000184466500005 |
| 公开日期 | 2011-07-08 |
| 源URL | [http://210.75.237.14/handle/351003/17385]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Jia, YH,Li, DS,Zhu, SW,et al. Characterization of a new bradykinin-potentiating peptide (TmF) from Trimeresurus mucrosquamatus[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(7):619_623. |
| APA | Jia, YH.,Li, DS.,Zhu, SW.,Zhang, LY.,Ding, LS.,...&Xiong, YL.(2003).Characterization of a new bradykinin-potentiating peptide (TmF) from Trimeresurus mucrosquamatus.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(7),619_623. |
| MLA | Jia, YH,et al."Characterization of a new bradykinin-potentiating peptide (TmF) from Trimeresurus mucrosquamatus".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.7(2003):619_623. |
入库方式： OAI收割
来源：成都生物研究所
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