Development of Fifty-one novel EST-SSR loci in the Pacific oyster, Crassostrea gigas by data mining from the public EST database
文献类型：期刊论文
| 作者 | Fu, DK; Yu, ZN; Wang, YH; carlzyu@scsio.ac.cn |
| 刊名 | CONSERVATION GENETICS RESOURCES
 |
| 出版日期 | 2010 |
| 卷号 | 2页码:13-18 |
| 关键词 | Crassostrea Gigas Est-ssr Development Polymorphism Cross-species Amplification |
| ISSN号 | 1877-7252 |
| 英文摘要 | Fifty-one novel EST-derived microsatellite markers were developed from 23,803 expressed sequence tags in the Pacific oyster, Crassostrea gigas. Polymorphisms of these loci were evaluated in a population of 48 individuals. The allele number ranges from 3 to 11, averaging 5.9 alleles per locus. The observed and expected heterozygosity values range from 0.023 to 0.875 (averaging 0.412) and 0.239 to 0.978 (averaging 0.681), respectively. Twenty-one of the 51 markers accord with Hardy-Weinberg equilibrium (HWE), and the rest show significant departure from HWE after Bonferroni correction, apparently due to heterozygote deficiency, suggesting likely occurrence of null alleles at loci and/or subpopulation of the sample. Cross-amplification was examined in C. hongkongensis and C. arikensis and the results show that 23 and 18 loci, respectively, have a reasonable polymorphism in the two species. These EST-SSR markers will be useful in studies of population genetics, linkage mapping and other aspects for this species. |
| URL标识 | 查看原文 |
| 资助项目 | LMB |
| WOS记录号 | WOS:000290413000004 |
| 公开日期 | 2011-07-02 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/3694]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | carlzyu@scsio.ac.cn |
| 推荐引用方式 GB/T 7714 | Fu, DK,Yu, ZN,Wang, YH,et al. Development of Fifty-one novel EST-SSR loci in the Pacific oyster, Crassostrea gigas by data mining from the public EST database[J]. CONSERVATION GENETICS RESOURCES,2010,2:13-18. |
| APA | Fu, DK,Yu, ZN,Wang, YH,&carlzyu@scsio.ac.cn.(2010).Development of Fifty-one novel EST-SSR loci in the Pacific oyster, Crassostrea gigas by data mining from the public EST database.CONSERVATION GENETICS RESOURCES,2,13-18. |
| MLA | Fu, DK,et al."Development of Fifty-one novel EST-SSR loci in the Pacific oyster, Crassostrea gigas by data mining from the public EST database".CONSERVATION GENETICS RESOURCES 2(2010):13-18. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。