PREPARATION OF LOW-MOLECULAR-MASS PEPTIDES BY ENZYME AUTOLYSIS OF Paphia undulata
文献类型：期刊论文
| 作者 | Chen, X; Sun, HL; Zhou, DB; fschenxin@tom.com |
| 刊名 | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 40期号:4页码:286-293 |
| 关键词 | Enzyme Autolysis Low-molecular-mass Peptides Optimum Condition Orthogonal Experiments Paphia Undulata Preparation |
| ISSN号 | 1082-6068 |
| 英文摘要 | Paphia undulata was used to prepare low-molecular-mass peptides by enzyme autolysis. The optimum condition for autolysis was studied to reach a maximum yield of peptides. Results showed that the yield of low-molecular-mass peptides was 4.33% and 78.57% of the total protein can be recovered under the optimized condition: pH=6.5, temperature=45 degrees C, reaction time=4h, ratio of starting material and water=1:3 and the enzyme dosage was 1374.0U/g. |
| 学科主题 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| URL标识 | 查看原文 |
| 资助项目 | LMB |
| WOS记录号 | WOS:000284629200006 |
| 公开日期 | 2011-07-02 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/3728]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | fschenxin@tom.com |
| 推荐引用方式 GB/T 7714 | Chen, X,Sun, HL,Zhou, DB,et al. PREPARATION OF LOW-MOLECULAR-MASS PEPTIDES BY ENZYME AUTOLYSIS OF Paphia undulata[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2010,40(4):286-293. |
| APA | Chen, X,Sun, HL,Zhou, DB,&fschenxin@tom.com.(2010).PREPARATION OF LOW-MOLECULAR-MASS PEPTIDES BY ENZYME AUTOLYSIS OF Paphia undulata.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,40(4),286-293. |
| MLA | Chen, X,et al."PREPARATION OF LOW-MOLECULAR-MASS PEPTIDES BY ENZYME AUTOLYSIS OF Paphia undulata".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 40.4(2010):286-293. |
入库方式： OAI收割
来源：南海海洋研究所
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