Microbiota of yellow grouper (Epinephelus awoora Temminck & Schlegel, 1842) fed two different diets
文献类型：期刊论文
| 作者 | Hu, CQ; cqhu@scsio.ac.cn; Chen, C; Zhang, LP; Luo, P; Feng, JB |
| 刊名 | AQUACULTURE RESEARCH
 |
| 出版日期 | 2010 |
| 卷号 | 41期号:12页码:1778-1790 |
| 关键词 | Yellow Grouper Microbiota Dgge Skin Gill Intestinal-non-adherent Bacteria Intestinal-adherent Bacteria |
| ISSN号 | 1355-557X |
| 英文摘要 | The rapidly growing yellow grouper industry has experienced relatively severe bacterial disease problems in China. The proliferation of pathogens in fish can be suppressed by commensal microbiota. In this background, we used nested polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and sequence analysis to investigate microbiota in the skin, gills and intestines, including adherent bacteria and non-adherent bacteria in yellow grouper fed with natural diet and complete feed. A total of 21 bacterial species were identified using phylogenetic analysis. The gamma-Proteobacteria group (81.0%, 17 species) dominated the bacterial communities in yellow grouper completely. Others belonged to Firmicutes (9.5%, two species), Actinobacteria (4.75%, one species) and Verrucomicrobia (4.75%, one species). The higher similarities (above 91%) of the DGGE band patterns in skin, gill and intestinal-non-adherent bacteria between two groups of fish indicated that existed more stable microbial communities existed in these specifically ecological niches in yellow grouper. However, considerable differences existed between two intestinal-adherent bacteria (IAB) samples; that is, compared with natural diet fed yellow grouper, higher bacterial apparent species richness and possibly less abundance existed in IAB in fish fed with complete diets, probably indicating that the community structures in IAB were affected easily and significantly by diet. |
| 学科主题 | Fisheries |
| URL标识 | 查看原文 |
| 资助项目 | LMB |
| WOS记录号 | WOS:000283946200006 |
| 公开日期 | 2011-07-02 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/3732]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | cqhu@scsio.ac.cn |
| 推荐引用方式 GB/T 7714 | Hu, CQ,cqhu@scsio.ac.cn,Chen, C,et al. Microbiota of yellow grouper (Epinephelus awoora Temminck & Schlegel, 1842) fed two different diets[J]. AQUACULTURE RESEARCH,2010,41(12):1778-1790. |
| APA | Hu, CQ,cqhu@scsio.ac.cn,Chen, C,Zhang, LP,Luo, P,&Feng, JB.(2010).Microbiota of yellow grouper (Epinephelus awoora Temminck & Schlegel, 1842) fed two different diets.AQUACULTURE RESEARCH,41(12),1778-1790. |
| MLA | Hu, CQ,et al."Microbiota of yellow grouper (Epinephelus awoora Temminck & Schlegel, 1842) fed two different diets".AQUACULTURE RESEARCH 41.12(2010):1778-1790. |
入库方式： OAI收割
来源：南海海洋研究所
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