Establishment and characterization of a new marine fish cell line derived from red-spotted grouper Epinephelus akaara
文献类型：期刊论文
| 作者 | qinqw@scsio.ac.cn; Sun, JJ; Gong, J; Huang, YH; Huang, EY; Huang, XH; Ou-Yang, ZL; Qin, QW |
| 刊名 | JOURNAL OF FISH BIOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 77期号:5页码:1083-1095 |
| 关键词 | Cell Line Epinephelus Akaara Grouper Liver Virus |
| ISSN号 | 0022-1112 |
| 英文摘要 | A new marine fish cell line, EAGL, derived from the liver of red-spotted grouper Epinephelus akaara was established and characterized. The cells multiplied well in minimum essential medium (MEM) supplemented with 10% foetal bovine serum (FBS) at temperatures between 25 and 30 degrees C. The growth rate of this cell line increased as the proportion of FBS increased from 5 to 20% at 25 degrees C, with maximum growth at the concentration of 15 or 20% FBS. Morphologically, the cells were epithelial-like and the presence of pancytokeratin confirmed their epithelial origin. Chromosome analysis revealed that the modal chromosome number was 48. The susceptibility of the cell line to four fish viruses was tested. Significant cytopathic effect (CPE) was only observed in Singapore grouper iridovirus (SGIV)-infected cells, and the virus replication was further confirmed by immunofluorescence, electron microscopy and real-time reverse-transcription (RT)-PCR assay. When the cells were transfected with pEGFP-N3 plasmid, bright fluorescent signals were observed, suggesting that this cell line can be used for transgenic and genetic manipulation studies. |
| 学科主题 | Fisheries ; Marine & Freshwater Biology |
| URL标识 | 查看原文 |
| 资助项目 | LMB |
| WOS记录号 | WOS:000282570600003 |
| 公开日期 | 2011-07-02 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/3748]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | qinqw@scsio.ac.cn |
| 推荐引用方式 GB/T 7714 | qinqw@scsio.ac.cn,Sun, JJ,Gong, J,et al. Establishment and characterization of a new marine fish cell line derived from red-spotted grouper Epinephelus akaara[J]. JOURNAL OF FISH BIOLOGY,2010,77(5):1083-1095. |
| APA | qinqw@scsio.ac.cn.,Sun, JJ.,Gong, J.,Huang, YH.,Huang, EY.,...&Qin, QW.(2010).Establishment and characterization of a new marine fish cell line derived from red-spotted grouper Epinephelus akaara.JOURNAL OF FISH BIOLOGY,77(5),1083-1095. |
| MLA | qinqw@scsio.ac.cn,et al."Establishment and characterization of a new marine fish cell line derived from red-spotted grouper Epinephelus akaara".JOURNAL OF FISH BIOLOGY 77.5(2010):1083-1095. |
入库方式： OAI收割
来源：南海海洋研究所
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