Genetic characterization of ticks from southwestern Romania by sequences of mitochondrial cox1 and nad5 genes
文献类型：期刊论文
| 作者 | Wu, XY; xingquanzh@scau.edu.cn; rqlin@scau.edu.cn; Zhu, XQ; Yuan, ZG; Song, HQ; Cosoroaba, I; Lin, RQ; Chitimia, L |
| 刊名 | EXPERIMENTAL AND APPLIED ACAROLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 52期号:3页码:305-311 |
| 关键词 | Ticks Cytochrome c Oxidase Subunit 1 Gene (Cox1) Nicotinamide Adenine Dinucleotide Dehydrogenase Subunit 5 Gene (Nad5) Phylogenetic Analysis Romania Mitochondrial Dna |
| ISSN号 | 0168-8162 |
| 英文摘要 | In the present study, samples representing three hard tick species and one soft tick species, namely Dermacentor marginatus, Haemaphysalis punctata, Ixodes ricinus and Argas persicus from southwestern Romania, and one hard tick, Haemaphysalis longicornis, from China were characterized genetically by a portion of mitochondrial cytochrome c oxidase subunit 1 gene (pcox1) and a portion of nicotinamide adenine dinucleotide dehydrogenase subunit 5 gene (pnad5). The pcox1 and pnad5 were amplified separately from individual ticks by PCR, sequenced and analyzed. The length of pcox1 and pnad5 sequences of all samples was 732 and 519 bp, respectively. The intra-specific sequence variation in De. marginatus was 0.1-1.0% for pcox1 and 0.2-1.2% for pnad5, whereas in Ha. punctata it was 0.4-1.9% for pcox1 and 0.4-1.0% for pnad5. For the tick species examined in the present study, sequence comparison revealed that the inter-specific sequence differences were higher: 15.9-27.6% for pcox1 and 20.3-42.4% for pnad5. This suggests that the cox1 and nad5 sequences could provide useful genetic markers for the specific identification and genetic characterization of ticks in Romania and elsewhere. |
| 学科主题 | Entomology |
| URL标识 | 查看原文 |
| 资助项目 | LMB |
| WOS记录号 | WOS:000282701300006 |
| 公开日期 | 2011-07-02 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/3752]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | xingquanzh@scau.edu.cn; rqlin@scau.edu.cn |
| 推荐引用方式 GB/T 7714 | Wu, XY,xingquanzh@scau.edu.cn,rqlin@scau.edu.cn,et al. Genetic characterization of ticks from southwestern Romania by sequences of mitochondrial cox1 and nad5 genes[J]. EXPERIMENTAL AND APPLIED ACAROLOGY,2010,52(3):305-311. |
| APA | Wu, XY.,xingquanzh@scau.edu.cn.,rqlin@scau.edu.cn.,Zhu, XQ.,Yuan, ZG.,...&Chitimia, L.(2010).Genetic characterization of ticks from southwestern Romania by sequences of mitochondrial cox1 and nad5 genes.EXPERIMENTAL AND APPLIED ACAROLOGY,52(3),305-311. |
| MLA | Wu, XY,et al."Genetic characterization of ticks from southwestern Romania by sequences of mitochondrial cox1 and nad5 genes".EXPERIMENTAL AND APPLIED ACAROLOGY 52.3(2010):305-311. |
入库方式： OAI收割
来源：南海海洋研究所
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