In silico cloning, expression of Rieske-like apoprotein gene and protein subcellular localization in the Pacific oyster, Crassostrea gigas
文献类型：期刊论文
| 作者 | Yu, ZN; He, XC; Zhang, Y; carlzyu@scsio.ac.cn |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2010 |
| 卷号 | 37期号:7页码:3259-3264 |
| 关键词 | Crassostrea Gigas In Silico clonIng Rieske Apoprotein Gene Expression Patterns Characterization Subcellular Localization |
| ISSN号 | 0301-4851 |
| 英文摘要 | Rieske protein gene in the Pacific oyster Crassostrea gigas was obtained by in silico cloning for the first time, and its expression profiles and subcellular localization were determined, respectively. The full-length cDNA of Cgisp is 985 bp in length and contains a 5'- and 3'-untranslated regions of 35 and 161 bp, respectively, with an open reading frame of 786 bp encoding a protein of 262 amino acids. The predicted molecular weight of 30 kDa of Cgisp protein was verified by prokaryotic expression. Conserved Rieske [2Fe-2S] cluster binding sites and highly matched-pair tertiary structure with 3CWB_E (Gallus gallus) were revealed by homologous analysis and molecular modeling. Eleven putative SNP sites and two conserved hexapeptide sequences, box I (THLGC) and II (PCHGS), were detected by multiple alignments. Real-time PCR analysis showed that Cgisp is expressed in a wide range of tissues, with adductor muscle exhibiting the top expression level, suggesting its biological function of energy transduction. The GFP tagging Cgisp indicated a mitochondrial localization, further confirming its physiological function. |
| 学科主题 | Biochemistry & Molecular Biology |
| URL标识 | 查看原文 |
| 资助项目 | LMB |
| WOS记录号 | WOS:000281985400028 |
| 公开日期 | 2011-07-02 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/3770]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | carlzyu@scsio.ac.cn |
| 推荐引用方式 GB/T 7714 | Yu, ZN,He, XC,Zhang, Y,et al. In silico cloning, expression of Rieske-like apoprotein gene and protein subcellular localization in the Pacific oyster, Crassostrea gigas[J]. MOLECULAR BIOLOGY REPORTS,2010,37(7):3259-3264. |
| APA | Yu, ZN,He, XC,Zhang, Y,&carlzyu@scsio.ac.cn.(2010).In silico cloning, expression of Rieske-like apoprotein gene and protein subcellular localization in the Pacific oyster, Crassostrea gigas.MOLECULAR BIOLOGY REPORTS,37(7),3259-3264. |
| MLA | Yu, ZN,et al."In silico cloning, expression of Rieske-like apoprotein gene and protein subcellular localization in the Pacific oyster, Crassostrea gigas".MOLECULAR BIOLOGY REPORTS 37.7(2010):3259-3264. |
入库方式： OAI收割
来源：南海海洋研究所
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