Differential profiles of gene expression in grouper Epinephelus coioides, infected with Singapore grouper iridovirus, revealed by suppression subtractive hybridization and DNA microarray
文献类型：期刊论文
| 作者 | Xu, D; Wei, J; Cui, H; Gong, J; Yan, Y; qinqw@scsio.ac.cn; Qin, Q; Lai, R |
| 刊名 | JOURNAL OF FISH BIOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 77期号:2页码:341-360 |
| 关键词 | Differential Gene Expression Epinephelus Coioides Microarray Singapore Grouper Iridovirus Suppression Subtractive Hybridization |
| ISSN号 | 0022-1112 |
| 英文摘要 | Suppression subtractive hybridization (SSH) was used to generate a subtracted cDNA library enriched with gene transcripts differentially expressed in the spleen of orange-spotted grouper Epinephelus coioides after 5 days of infection with Singapore grouper iridovirus (SGIV). In the forward and reverse-subtracted libraries, 260 and 153 non-redundant expressed sequence tags (EST), respectively, were identified. These annotated genes responding to SGIV infection were grouped into eight gene categories related to immunity, cell structure, transcription-translation, cell signalling, metabolism, mitochondrial proteins, ribosomal proteins and unknown or hypothetical proteins. A DNA microarray containing all the differentially expressed genes was constructed, and the gene expression patterns in different tissues were investigated in virus-infected E. coioides. Of these genes, four associated with the infection processes were identified and further investigated by quantitative real-time PCR. These results provide new insights into the molecular basis of host-pathogen interactions in E. coioides, and will help the development of control strategies against SGIV infection. |
| 学科主题 | Fisheries ; Marine & Freshwater Biology |
| URL标识 | 查看原文 |
| 资助项目 | LMB |
| WOS记录号 | WOS:000280151300003 |
| 公开日期 | 2011-07-02 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/3794]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | qinqw@scsio.ac.cn |
| 推荐引用方式 GB/T 7714 | Xu, D,Wei, J,Cui, H,et al. Differential profiles of gene expression in grouper Epinephelus coioides, infected with Singapore grouper iridovirus, revealed by suppression subtractive hybridization and DNA microarray[J]. JOURNAL OF FISH BIOLOGY,2010,77(2):341-360. |
| APA | Xu, D.,Wei, J.,Cui, H.,Gong, J.,Yan, Y.,...&Lai, R.(2010).Differential profiles of gene expression in grouper Epinephelus coioides, infected with Singapore grouper iridovirus, revealed by suppression subtractive hybridization and DNA microarray.JOURNAL OF FISH BIOLOGY,77(2),341-360. |
| MLA | Xu, D,et al."Differential profiles of gene expression in grouper Epinephelus coioides, infected with Singapore grouper iridovirus, revealed by suppression subtractive hybridization and DNA microarray".JOURNAL OF FISH BIOLOGY 77.2(2010):341-360. |
入库方式： OAI收割
来源：南海海洋研究所
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