Identification and characterization of Singapore grouper iridovirus (SGIV) ORF162L, an immediate-early gene involved in cell growth control and viral replication
文献类型：期刊论文
| 作者 | Xia, LQ; Liang, HY; Huang, YH; Ou-Yang, ZL; Qin, QW; qinqw@scsio.ac.cn |
| 刊名 | VIRUS RESEARCH
 |
| 出版日期 | 2010 |
| 卷号 | 147期号:1页码:30-39 |
| 关键词 | Iridovirus Sgiv Icp46 Structural Protein Cell Growth Viral Replication |
| ISSN号 | 0168-1702 |
| 英文摘要 | Singapore grouper iridovirus (SGIV) is a major pathogen resulting in heavy economic losses to grouper aquaculture. In this study, SGIV ORF162L encoding a putative homolog of ICP46 was identified and characterized. Interestingly, ICP46 could be found in all sequenced iridoviruses and is considered as a core gene of the family Iridoviridae. SGIV ICP46 was classified as an immediate-early (IE) gene during in vitro infection using drug inhibition analysis, reverse transcription polymerase chain reaction and Western blot analysis. Subcellular localization revealed that SGIV ICP46 was distributed predominantly in the cytoplasm. Furthermore, SGIV ICP46 proved to be a structural protein of the nucleocapsid; its overexpression could promote the growth of grouper embryonic cells and contribute to SGIV replication. This is the first report of the characterization of a putative ICP46 homolog and these results should offer important insights into the pathogenesis of iridoviruses. (c) 2009 Elsevier B.V. All rights reserved. |
| 学科主题 | Virology |
| URL标识 | 查看原文 |
| 资助项目 | LMB |
| WOS记录号 | WOS:000276560300005 |
| 公开日期 | 2011-07-02 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/3858]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | qinqw@scsio.ac.cn |
| 推荐引用方式 GB/T 7714 | Xia, LQ,Liang, HY,Huang, YH,et al. Identification and characterization of Singapore grouper iridovirus (SGIV) ORF162L, an immediate-early gene involved in cell growth control and viral replication[J]. VIRUS RESEARCH,2010,147(1):30-39. |
| APA | Xia, LQ,Liang, HY,Huang, YH,Ou-Yang, ZL,Qin, QW,&qinqw@scsio.ac.cn.(2010).Identification and characterization of Singapore grouper iridovirus (SGIV) ORF162L, an immediate-early gene involved in cell growth control and viral replication.VIRUS RESEARCH,147(1),30-39. |
| MLA | Xia, LQ,et al."Identification and characterization of Singapore grouper iridovirus (SGIV) ORF162L, an immediate-early gene involved in cell growth control and viral replication".VIRUS RESEARCH 147.1(2010):30-39. |
入库方式： OAI收割
来源：南海海洋研究所
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