Identification and characterization of a novel capsid protein encoded by Singapore grouper iridovirus ORF038L
文献类型：期刊论文
| 作者 | Wan, QJ; Gong, J; Huang, XH; Huang, YH; Zhou, S; Ou-Yang, ZL; Cao, JH; Ye, LL; Qin, QW; qinqw@scsio.ac.cn |
| 刊名 | ARCHIVES OF VIROLOGY
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| 出版日期 | 2010 |
| 卷号 | 155期号:3页码:351-359 |
| ISSN号 | 0304-8608 |
| 英文摘要 | Singapore grouper iridovirus (SGIV) is an important pathogen isolated from grouper, Epinephelus tauvina, and characterized as a novel ranavirus. To better understand the function of viral structural genes involved in SGIV infection and virus-host interactions, a candidate gene, VP38 (ORF038L), was investigated in this study. SGIV VP38 was found to encode a 170-aa peptide containing an RGD motif, and it showed significant identity only to members of the genus Iridovirus, family Iridoviridae, except megalocytivirus. The VP38 gene was identified by temporal expression pattern analysis and drug inhibition assay as a late (L) gene. Immunofluorescence localization revealed that P38 was distributed predominately in the cytoplasm and that association of VP38 with viral factories increased at the late stage of SGIV infection. Consistent results from immunoelectron microscopy (IEM) and western blot analysis revealed that SGIV VP38 is a viral capsid protein. Furthermore, antibodies specific for SGIV VP38 exhibited substantial SGIV-neutralizing activity in vitro, suggesting that VP38 might play an important role in SGIV infectivity. |
| 学科主题 | Virology |
| URL标识 | 查看原文 |
| 资助项目 | LMB |
| WOS记录号 | WOS:000274939600007 |
| 公开日期 | 2011-07-02 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/3900]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | qinqw@scsio.ac.cn |
| 推荐引用方式 GB/T 7714 | Wan, QJ,Gong, J,Huang, XH,et al. Identification and characterization of a novel capsid protein encoded by Singapore grouper iridovirus ORF038L[J]. ARCHIVES OF VIROLOGY,2010,155(3):351-359. |
| APA | Wan, QJ.,Gong, J.,Huang, XH.,Huang, YH.,Zhou, S.,...&qinqw@scsio.ac.cn.(2010).Identification and characterization of a novel capsid protein encoded by Singapore grouper iridovirus ORF038L.ARCHIVES OF VIROLOGY,155(3),351-359. |
| MLA | Wan, QJ,et al."Identification and characterization of a novel capsid protein encoded by Singapore grouper iridovirus ORF038L".ARCHIVES OF VIROLOGY 155.3(2010):351-359. |
入库方式： OAI收割
来源：南海海洋研究所
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