Molecular cloning, characterization and expression analysis of a C-type lectin (Ec-CTL) in orange-spotted grouper, Epinephelus coioides
文献类型：期刊论文
| 作者 | Wei, JG; Xu, D; Zhou, JG; Cui, HC; Yan, Y; Ouyang, ZL; Gong, J; Huang, YH; Huang, XH; Qjn, QW |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 28期号:1页码:178-186 |
| ISSN号 | 1050-4648 |
| 关键词 | Epinephelus Coioides Singapore Grouper Iridovirus (Sgiv) C-type Lectin Molecular Cloning Expression |
| 英文摘要 | C-type lectins play crucial roles in pathogen recognition, innate immunity, and cell-cell interactions. In this study, a new C-type lectin (Ec-CTL) gene was cloned from grouper, Epinephelus coioides by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of Ec-CTL was composed of 840 bp with a 651 bp open reading frame (ORF) that encodes a 216-residue protein. The deduced amino acid sequence of Ec-CTL possessed all conserved features crucial for the fundamental structure, such as the four cysteine residues (Cys(71), Cys(152), Cys(167), Cys(175)) involved in the formation of disulphide bridges and the potential Ca2+/carbohydrate-binding sites. Ec-CTL contains a signal peptide and a single carbohydrate recognition domain (CRD). The genomic DNA of the gene consists of three exons and two introns. Ec-CTL showed high similarity of 54% to the C-type lectin of killifish Fundulus heteroclitus. Ec-CTL mRNA is predominately expressed in liver and skin, and lower expressed in kidney, intestine, heart, brain and spleen. The expression of Ec-CTL was differentially up-regulated in orange-spotted grouper challenged with Saccharomyces cerevisiae, Vibrio vulnificus, Staphyloccocus aureus and Singapore grouper iridovirus (SGIV). Recombinant mature Ec-CTL (rEc-CTL) was expressed in E. coli BL21, purified and characterized as a typical Ca2+-dependent carbohydrate-binding protein possessing hemagglutinating activity. It bound to all examined bacterial and yeast strains, and aggregated with S. cerevisiae, V vulnificus and S. aureus in a Ca2+-dependent manner. (C) 2009 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| URL标识 | 查看原文 |
| 资助项目 | LMB |
| WOS记录号 | WOS:000274235100022 |
| 公开日期 | 2011-07-02 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/3904]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | qinqw@scsio.ac.cn |
| 推荐引用方式 GB/T 7714 | Wei, JG,Xu, D,Zhou, JG,et al. Molecular cloning, characterization and expression analysis of a C-type lectin (Ec-CTL) in orange-spotted grouper, Epinephelus coioides[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(1):178-186. |
| APA | Wei, JG.,Xu, D.,Zhou, JG.,Cui, HC.,Yan, Y.,...&qinqw@scsio.ac.cn.(2010).Molecular cloning, characterization and expression analysis of a C-type lectin (Ec-CTL) in orange-spotted grouper, Epinephelus coioides.FISH & SHELLFISH IMMUNOLOGY,28(1),178-186. |
| MLA | Wei, JG,et al."Molecular cloning, characterization and expression analysis of a C-type lectin (Ec-CTL) in orange-spotted grouper, Epinephelus coioides".FISH & SHELLFISH IMMUNOLOGY 28.1(2010):178-186. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。