Phylogenetic diversity of nitrogen-fixing bacteria in mangrove sediments assessed by PCR-denaturing gradient gel electrophoresis
文献类型：期刊论文
| 作者 | Wang, YS; Zhang, YY; Dong, JD; Yang, ZH; Zhang, S; amy0517@gmail.com |
| 刊名 | ARCHIVES OF MICROBIOLOGY
 |
| 出版日期 | 2008 |
| 卷号 | 190期号:1页码:19-28 |
| ISSN号 | 0302-8933 |
| 关键词 | Mangrove Sediments Nitrogen-fixing Bacteria Denaturing Gradient Gel Electrophoresis Multivariate Analysis |
| 英文摘要 | Culture-independent PCR-denaturing gradient gel electrophoresis (DGGE) was employed to assess the composition of diazotroph species from the sediments of three mangrove ecosystem sites in Sanya, Hainan Island, China. A strategy of removing humic acids prior to DNA extraction was conducted, then total community DNA was extracted using the soil DNA kit successfully for nifH PCR amplification, which simplified the current procedure and resulted in good DGGE profiles. The results revealed a novel nitrogen-fixing bacterial profile and fundamental diazotrophic biodiversity in mangrove sediments, as reflected by the numerous bands present DGGE patterns. Canonical correspondence analysis (CCA) revealed that the sediments organic carbon concentration and available soil potassium accounted for a significant amount of the variability in the nitrogen-fixing bacterial community composition. The predominant DGGE bands were sequenced, yielding 31 different nifH sequences, which were used in phylogenetic reconstructions. Most sequences were from Proteobacteria, e.g. alpha, gamma, beta, delta-subdivisions, and characterized by sequences of members of genera Azotobacter, Desulfuromonas, Sphingomonas, Geobacter, Pseudomonas, Bradyrhizobium and Derxia. These results significantly expand our knowledge of the nitrogen-fixing bacterial diversity of the mangrove environment. |
| 学科主题 | Microbiology |
| URL标识 | 查看原文 |
| 资助项目 | LMB |
| WOS记录号 | WOS:000256927000003 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/4982]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | amy0517@gmail.com |
| 推荐引用方式 GB/T 7714 | Wang, YS,Zhang, YY,Dong, JD,et al. Phylogenetic diversity of nitrogen-fixing bacteria in mangrove sediments assessed by PCR-denaturing gradient gel electrophoresis[J]. ARCHIVES OF MICROBIOLOGY,2008,190(1):19-28. |
| APA | Wang, YS,Zhang, YY,Dong, JD,Yang, ZH,Zhang, S,&amy0517@gmail.com.(2008).Phylogenetic diversity of nitrogen-fixing bacteria in mangrove sediments assessed by PCR-denaturing gradient gel electrophoresis.ARCHIVES OF MICROBIOLOGY,190(1),19-28. |
| MLA | Wang, YS,et al."Phylogenetic diversity of nitrogen-fixing bacteria in mangrove sediments assessed by PCR-denaturing gradient gel electrophoresis".ARCHIVES OF MICROBIOLOGY 190.1(2008):19-28. |
入库方式： OAI收割
来源：南海海洋研究所
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