Bioinformatic mining of EST-SSR loci in the Pacific oyster, Crassostrea gigas
文献类型：期刊论文
| 作者 | Yu, Z; Wang, Y; Ren, R; carlzyu@scsio.ac.cn |
| 刊名 | ANIMAL GENETICS
 |
| 出版日期 | 2008 |
| 卷号 | 39期号:3页码:287-289 |
| 关键词 | Crassostrea Gigas Cross-species Amplification Est-ssr Microsatellite |
| ISSN号 | 0268-9146 |
| 英文摘要 | A set of expressed sequence tag-simple sequence repeat (EST-SSR) markers of the Pacific oyster, Crassostrea gigas, was developed through bioinformatic mining of the GenBank public database. As of June 30, 2007, a total of 5132 EST sequences from GenBank were downloaded and screened for di-, tri- and tetra-nucleotide repeats, with criteria set at a minimum of 5, 4 and 4 repeats for the three categories of SSRs respectively. Seventeen polymorphic microsatellite markers were characterized. Allele numbers ranged from 3 to 10, and the observed and expected heterozygosity values varied from 0.125 to 0.770 and from 0.113 to 0.732 respectively. Eleven loci were at Hardy-Weinberg equilibrium (HWE); the other six loci showed significant departure from HWE (P < 0.01), suggesting possible presence of null alleles. Pairwise check of linkage disequilibrium (LD) indicated that 11 of 136 pairs of loci showed significant LD (P < 0.01), likely due to HWE present in single markers. Cross-species amplification was examined for five other Crassostrea species and reasonable results were obtained, promising usefulness of these markers in oyster genetics. |
| 学科主题 | Agriculture, Dairy & Animal Science ; Genetics & Heredity |
| URL标识 | 查看原文 |
| 资助项目 | LMB |
| WOS记录号 | WOS:000255475700011 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/5002]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | carlzyu@scsio.ac.cn |
| 推荐引用方式 GB/T 7714 | Yu, Z,Wang, Y,Ren, R,et al. Bioinformatic mining of EST-SSR loci in the Pacific oyster, Crassostrea gigas[J]. ANIMAL GENETICS,2008,39(3):287-289. |
| APA | Yu, Z,Wang, Y,Ren, R,&carlzyu@scsio.ac.cn.(2008).Bioinformatic mining of EST-SSR loci in the Pacific oyster, Crassostrea gigas.ANIMAL GENETICS,39(3),287-289. |
| MLA | Yu, Z,et al."Bioinformatic mining of EST-SSR loci in the Pacific oyster, Crassostrea gigas".ANIMAL GENETICS 39.3(2008):287-289. |
入库方式： OAI收割
来源：南海海洋研究所
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