Immunogenicity and protective effects of inactivated Singapore grouper iridovirus (SGIV) vaccines in orange-spotted grouper, Epinephelus coioides
文献类型：期刊论文
| 作者 | Qin, QW; Ou-Yang, ZL; Wang, PR; Huang, XH; Cai, J; Huang, YH; Wei, SN; Ji, HS; Wei, JG; Zhou, YC |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 38期号:2页码:254-261 |
| 关键词 | Singapore Grouper Iridovirus (Sgiv) Inactivated Vaccine Formalin Beta-propiolactone Elisa Immune-related Genes |
| ISSN号 | 0145-305X |
| 英文摘要 | Vaccination is one of the best methods against viral diseases. In this study, experimental inactivated Singapore grouper iridovirus (SGIV) vaccines were prepared, and immunogenicity and protection against virus infection of the vaccines were investigated in orange-spotted grouper, Epinephelus coioides. Two kinds of vaccines, including,beta-propiolactone (BPL) inactivated virus at 4 degrees C for 12 h and formalin inactivated virus at 4 degrees C for 12 d, was highly protective against the challenge at 30-day post-vaccination and produced relative percent of survival rates of 91.7% and 100%, respectively. These effective vaccinations induced potent innate immune responses mediated by pro-inflammatory cytokines and type! interferon (IFN)-stimulated genes (ISGs). It is noteworthy that ISGs, such as Mx and ISG15, were up-regulated only in the effective vaccine groups, which suggested that type 1 IFN system may be the functional basis of early anti-viral immunity. Moreover, effective vaccination also significantly up-regulated of the expression of MHC class I gene and produced substantial amount of specific serum antibody at 4 weeks post-vaccination. Taken together, our results clearly demonstrated that effective vaccination in grouper induced an early, nonspecific antiviral immunity, and later, a specific immune response involving both humoral and cell-mediated immunity. (C) 2012 Elsevier Ltd. All rights reserved. |
| 学科主题 | Immunology ; Zoology |
| 资助项目 | LMB |
| 语种 | 英语 |
| WOS记录号 | WOS:000309312700007 |
| 公开日期 | 2013-08-28 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/9792]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | qinqw@scsio.ac.cn |
| 推荐引用方式 GB/T 7714 | Qin, QW,Ou-Yang, ZL,Wang, PR,et al. Immunogenicity and protective effects of inactivated Singapore grouper iridovirus (SGIV) vaccines in orange-spotted grouper, Epinephelus coioides[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,38(2):254-261. |
| APA | Qin, QW.,Ou-Yang, ZL.,Wang, PR.,Huang, XH.,Cai, J.,...&qinqw@scsio.ac.cn.(2012).Immunogenicity and protective effects of inactivated Singapore grouper iridovirus (SGIV) vaccines in orange-spotted grouper, Epinephelus coioides.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,38(2),254-261. |
| MLA | Qin, QW,et al."Immunogenicity and protective effects of inactivated Singapore grouper iridovirus (SGIV) vaccines in orange-spotted grouper, Epinephelus coioides".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 38.2(2012):254-261. |
入库方式： OAI收割
来源：南海海洋研究所
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